Supplementary information
From the following article
Homomultimeric complexes of CD22 in B cells revealed by protein-glycan cross-linking
Shoufa Han, Brian E Collins, Per Bengtson & James C Paulson
Nature Chemical Biology 1, 93 - 97 (2005) Published online: 12 June 2005
doi:10.1038/nchembio713
Supplementary Fig. 1
Requirement of UV light for crosslinking of CD22 in situ.
Supplementary Fig. 2
Demonstration of equivalent biotinylation of cell preparations used for SNA and CD22-Fc immunoprecipitations in.
Supplementary Fig. 3
CD45, CD19, and PMCA can be immunoprecipitated by CD22-Fc in a sialic acid dependent manner.
Supplementary Fig. 4
Absence of CD22 crosslinking to CD19 or CD45.
Supplementary Fig. 5
Comparison of CD22 crosslinking in resting and activated K20 cells.
Supplementary Fig. 6
CD22 interacts in cis with glycans on neighboring CD22 molecules to form homo-multimeric complexes.
Supplementary Methods
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