Latest Research

  • Article |

    Boyd et al. monitored the effects of patient-derived acute myeloid leukaemia (AML) cells on human HSPCs in vivo and found that AML impairs bone marrow adipocyte differentiation, and this in turn impedes healthy endogenous haematopoiesis.

    • Allison L. Boyd
    • , Jennifer C. Reid
    • , Kyle R. Salci
    • , Lili Aslostovar
    • , Yannick D. Benoit
    • , Zoya Shapovalova
    • , Mio Nakanishi
    • , Deanna P. Porras
    • , Mohammed Almakadi
    • , Clinton J. V. Campbell
    • , Michael F. Jackson
    • , Catherine A. Ross
    • , Ronan Foley
    • , Brian Leber
    • , David S. Allan
    • , Mitchell Sabloff
    • , Anargyros Xenocostas
    • , Tony J. Collins
    •  & Mickie Bhatia
  • Letter |

    Rondinelli et al. show that EZH2-mediated H3K27me3 at stalled replication forks recruits MUS81 nuclease to facilitate fork degradation. Loss of EZH2 contributes to PARPi resistance in BRCA2-deficient tumours.

    • Beatrice Rondinelli
    • , Ewa Gogola
    • , Hatice Yücel
    • , Alexandra A. Duarte
    • , Marieke van de Ven
    • , Roxanne van der Sluijs
    • , Panagiotis A. Konstantinopoulos
    • , Jos Jonkers
    • , Raphaël Ceccaldi
    • , Sven Rottenberg
    •  & Alan D. D’Andrea
  • Article |

    Gilot et al. have found that TYRP1 mRNA, in addition to coding for TYRP1 protein, can promote melanoma by sequestering the tumour suppressor miR-16, thus de-repressing the mRNA transcription of miR-16 target RAB17, which is involved in melanoma cell proliferation.

    • David Gilot
    • , Mélodie Migault
    • , Laura Bachelot
    • , Fabrice Journé
    • , Aljosja Rogiers
    • , Emmanuelle Donnou-Fournet
    • , Ariane Mogha
    • , Nicolas Mouchet
    • , Marie-Laure Pinel-Marie
    • , Bernard Mari
    • , Tristan Montier
    • , Sébastien Corre
    • , Arthur Gautron
    • , Florian Rambow
    • , Petra El Hajj
    • , Rania Ben Jouira
    • , Sophie Tartare-Deckert
    • , Jean-Christophe Marine
    • , Brice Felden
    • , Ghanem Ghanem
    •  & Marie-Dominique Galibert
  • Article |

    Wu et al. show that prosurvival BCL-2 proteins disrupt SUFU repression of GLI activity, leading to GLI target gene expression. BH3 mimetics disable GLI transcriptional activity driven by HH pathway mutations.

    • Xiaofeng Wu
    • , Li-shu Zhang
    • , Jason Toombs
    • , Yi-Chun Kuo
    • , John Tyler Piazza
    • , Rubina Tuladhar
    • , Quinn Barrett
    • , Chih-wei Fan
    • , Xuewu Zhang
    • , Loren D. Walensky
    • , Marcel Kool
    • , Steven Y. Cheng
    • , Rolf Brekken
    • , Joseph T. Opferman
    • , Douglas R. Green
    • , Tudor Moldoveanu
    •  & Lawrence Lum
  • Article |

    Anderson et al. show that LIF, ActA and Wnt support self-renewal in both pluripotent and endodermal cells. Upon removal of insulin, they induce differentiation of naive PSCs into extra-embryonic primitive endoderm and primed PSCs into definitive endoderm.

    • Kathryn G. V. Anderson
    • , William B. Hamilton
    • , Fabian V. Roske
    • , Ajuna Azad
    • , Teresa E. Knudsen
    • , Maurice A. Canham
    • , Lesley M. Forrester
    •  & Joshua M. Brickman
  • Article |

    Dondelinger et al. and Menon et al. show that MAPKAP kinase-2 (MK2) phosphorylates RIPK1 to regulate TNF-mediated cell death as well as RIPK1 signalling in inflammation and bacterial infection.

    • Yves Dondelinger
    • , Tom Delanghe
    • , Diego Rojas-Rivera
    • , Dario Priem
    • , Tinneke Delvaeye
    • , Inge Bruggeman
    • , Franky Van Herreweghe
    • , Peter Vandenabeele
    •  & Mathieu J. M. Bertrand
  • Article |

    Dondelinger et al. and Menon et al. show that MAPKAP kinase-2 (MK2) phosphorylates RIPK1 to regulate TNF-mediated cell death as well as RIPK1 signalling in inflammation and bacterial infection.

    • Manoj B. Menon
    • , Julia Gropengießer
    • , Jessica Fischer
    • , Lena Novikova
    • , Anne Deuretzbacher
    • , Juri Lafera
    • , Hanna Schimmeck
    • , Nicole Czymmeck
    • , Natalia Ronkina
    • , Alexey Kotlyarov
    • , Martin Aepfelbacher
    • , Matthias Gaestel
    •  & Klaus Ruckdeschel
  • Article |

    Ganuza et al. track newly specified blood progenitors in the dorsal aorta of the mouse embryo and demonstrate that they are polyclonal in origin and that hundreds of mesodermal, endothelial and blood precursors establish lifelong haematopoiesis.

    • Miguel Ganuza
    • , Trent Hall
    • , David Finkelstein
    • , Ashley Chabot
    • , Guolian Kang
    •  & Shannon McKinney-Freeman
  • Article |

    Wu et al. find that tumour hypoxic conditions increase miR25/93 levels, which via targeting Ncoa3 downregulate the expression of the innate immune regulator cGAS, thus allowing escape of the anti-tumour immune response.

    • Min-Zu Wu
    • , Wei-Chung Cheng
    • , Su-Feng Chen
    • , Shin Nieh
    • , Carolyn O’Connor
    • , Chia-Lin Liu
    • , Wen-Wei Tsai
    • , Cheng-Jang Wu
    • , Lorena Martin
    • , Yaoh-Shiang Lin
    • , Kou-Juey Wu
    • , Li-Fan Lu
    •  & Juan Carlos Izpisua Belmonte
  • Article |

    McNally et al. identify the retriever complex as required for endosomal cargo recycling. Retriever binds SNX17, the CCC and WASH complexes to govern cell surface expression of integrins, receptors and transporters.

    • Kerrie E. McNally
    • , Rebecca Faulkner
    • , Florian Steinberg
    • , Matthew Gallon
    • , Rajesh Ghai
    • , David Pim
    • , Paul Langton
    • , Neil Pearson
    • , Chris M. Danson
    • , Heike Nägele
    • , Lindsey L. Morris
    • , Amika Singla
    • , Brittany L. Overlee
    • , Kate J. Heesom
    • , Richard Sessions
    • , Lawrence Banks
    • , Brett M. Collins
    • , Imre Berger
    • , Daniel D. Billadeau
    • , Ezra Burstein
    •  & Peter J. Cullen
  • Article |

    Lee et al. found that the ubiquitin-editing enzyme A20 promotes TGF-β1-induced EMT and metastases of breast cancer cells via ubiquitylation-mediated nuclear stabilization of Snail1.

    • Ji-Hyung Lee
    • , Su Myung Jung
    • , Kyung-Min Yang
    • , Eunjin Bae
    • , Sung Gwe Ahn
    • , Jin Seok Park
    • , Dongyeob Seo
    • , Minbeom Kim
    • , Jihoon Ha
    • , Jaewon Lee
    • , Jun-Hyeong Kim
    • , Jun Hwan Kim
    • , Akira Ooshima
    • , Jinah Park
    • , Donghyuk Shin
    • , Youn Sook Lee
    • , Sangho Lee
    • , Geert van Loo
    • , Joon Jeong
    • , Seong-Jin Kim
    •  & Seok Hee Park
  • Article |

    Tait and colleagues show that caspase-independent cell death induced by mitochondrial permeabilization stimulates NF-κB activity through downregulation of inhibitor of apoptosis, and enhances anti-tumour effects.

    • Evangelos Giampazolias
    • , Barbara Zunino
    • , Sandeep Dhayade
    • , Florian Bock
    • , Catherine Cloix
    • , Kai Cao
    • , Alba Roca
    • , Jonathan Lopez
    • , Gabriel Ichim
    • , Emma Proïcs
    • , Camila Rubio-Patiño
    • , Loic Fort
    • , Nader Yatim
    • , Emma Woodham
    • , Susana Orozco
    • , Lucia Taraborrelli
    • , Nieves Peltzer
    • , Daniele Lecis
    • , Laura Machesky
    • , Henning Walczak
    • , Matthew L. Albert
    • , Simon Milling
    • , Andrew Oberst
    • , Jean-Ehrland Ricci
    • , Kevin M. Ryan
    • , Karen Blyth
    •  & Stephen W. G. Tait
  • Article |

    Skamagki et al. show that pluripotency factor ZSCAN10 is poorly expressed in iPSCs derived from aged donors, and its addition during reprogramming restores the DNA damage response and genomic stability through normalization of ROS–glutathione levels.

    • Maria Skamagki
    • , Cristina Correia
    • , Percy Yeung
    • , Timour Baslan
    • , Samuel Beck
    • , Cheng Zhang
    • , Christian A. Ross
    • , Lam Dang
    • , Zhong Liu
    • , Simona Giunta
    • , Tzu-Pei Chang
    • , Joye Wang
    • , Aparna Ananthanarayanan
    • , Martina Bohndorf
    • , Benedikt Bosbach
    • , James Adjaye
    • , Hironori Funabiki
    • , Jonghwan Kim
    • , Scott Lowe
    • , James J. Collins
    • , Chi-Wei Lu
    • , Hu Li
    • , Rui Zhao
    •  & Kitai Kim
  • Article |

    Shi et al. map the ciliary transition zone by STORM imaging, characterizing protein arrangements in nested rings and finding that mutations in RPGRIP1L that are associated with the ciliopathy Joubert syndrome disrupt SMO ciliary localization.

    • Xiaoyu Shi
    • , Galo Garcia III
    • , Julie C. Van De Weghe
    • , Ryan McGorty
    • , Gregory J. Pazour
    • , Dan Doherty
    • , Bo Huang
    •  & Jeremy F. Reiter
  • Article |

    Zihni et al. discover a role for Cdc42–MRCK signalling in establishing contractility at the apical pole, which in turn controls epithelial polarity in mammalian cells and Drosophila photoreceptors.

    • Ceniz Zihni
    • , Evi Vlassaks
    • , Stephen Terry
    • , Jeremy Carlton
    • , Thomas King Chor Leung
    • , Michael Olson
    • , Franck Pichaud
    • , Maria Susana Balda
    •  & Karl Matter
  • Article |

    Schalbetter et al. show by Hi-C and modelling that mitotic chromosome compaction in budding yeast occurs by cis-looping of chromatin, and reveal distinct roles for cohesin and condensin depending on chromatin context.

    • Stephanie Andrea Schalbetter
    • , Anton Goloborodko
    • , Geoffrey Fudenberg
    • , Jon-Matthew Belton
    • , Catrina Miles
    • , Miao Yu
    • , Job Dekker
    • , Leonid Mirny
    •  & Jonathan Baxter
  • Article |

    Grelet et al. find that hnRNP E1 release from PNUTS pre-RNA in response to TGFβ generates a lncRNA that acts as competitive sponge for miR-205, promoting epithelial–mesenchymal transition in cancer.

    • Simon Grelet
    • , Laura A. Link
    • , Breege Howley
    • , Clémence Obellianne
    • , Viswanathan Palanisamy
    • , Vamsi K. Gangaraju
    • , J. Alan Diehl
    •  & Philip H. Howe
  • Article |

    Horton et al. show that early Crebbp loss in haematopoietic progenitors results in a defective p53-mediated DNA damage response, leading to the accumulation of epigenetic and genetic alterations, which promote the onset of lymphoid malignancies.

    • Sarah J. Horton
    • , George Giotopoulos
    • , Haiyang Yun
    • , Shabana Vohra
    • , Olivia Sheppard
    • , Rachael Bashford-Rogers
    • , Mamunur Rashid
    • , Alexandra Clipson
    • , Wai-In Chan
    • , Daniel Sasca
    • , Loukia Yiangou
    • , Hikari Osaki
    • , Faisal Basheer
    • , Paolo Gallipoli
    • , Natalie Burrows
    • , Ayşegül Erdem
    • , Anastasiya Sybirna
    • , Sarah Foerster
    • , Wanfeng Zhao
    • , Tonci Sustic
    • , Anna Petrunkina Harrison
    • , Elisa Laurenti
    • , Jessica Okosun
    • , Daniel Hodson
    • , Penny Wright
    • , Ken G. Smith
    • , Patrick Maxwell
    • , Jude Fitzgibbon
    • , Ming Q. Du
    • , David J. Adams
    •  & Brian J. P. Huntly
  • Article |

    Hiraike et al. identify nuclear factor I-A (NFIA) as a transcriptional regulator of brown fat. NFIA activates cell-type-specific enhancers prior to differentiation and facilitates PPARγ binding to regulate the brown fat gene program.

    • Yuta Hiraike
    • , Hironori Waki
    • , Jing Yu
    • , Masahiro Nakamura
    • , Kana Miyake
    • , Gaku Nagano
    • , Ryo Nakaki
    • , Ken Suzuki
    • , Hirofumi Kobayashi
    • , Shogo Yamamoto
    • , Wei Sun
    • , Tomohisa Aoyama
    • , Yusuke Hirota
    • , Haruya Ohno
    • , Kenji Oki
    • , Masayasu Yoneda
    • , Andrew P. White
    • , Yu-Hua Tseng
    • , Aaron M. Cypess
    • , Therese J. Larsen
    • , Naja Z. Jespersen
    • , Camilla Scheele
    • , Shuichi Tsutsumi
    • , Hiroyuki Aburatani
    • , Toshimasa Yamauchi
    •  & Takashi Kadowaki
  • Article |

    Flores et al. show that hair follicle stem cells rely on the production of lactate via the LDHA enzyme to become activated. Inducing Ldha through Mpc1 inhibition or Myc activation successfully reactivates the hair cycle in quiescent follicles.

    • Aimee Flores
    • , John Schell
    • , Abigail S. Krall
    • , David Jelinek
    • , Matilde Miranda
    • , Melina Grigorian
    • , Daniel Braas
    • , Andrew C. White
    • , Jessica L. Zhou
    • , Nicholas A. Graham
    • , Thomas Graeber
    • , Pankaj Seth
    • , Denis Evseenko
    • , Hilary A. Coller
    • , Jared Rutter
    • , Heather R. Christofk
    •  & William E. Lowry
  • Article |

    Schell et al. demonstrate that inactivation of the mitochondrial pyruvate carrier in mouse and fly intestinal stem cells (ISCs) locks the cell into a glycolytic metabolic program and promotes the expansion of the stem cell compartment.

    • John C. Schell
    • , Dona R. Wisidagama
    • , Claire Bensard
    • , Helong Zhao
    • , Peng Wei
    • , Jason Tanner
    • , Aimee Flores
    • , Jeffrey Mohlman
    • , Lise K. Sorensen
    • , Christian S. Earl
    • , Kristofor A. Olson
    • , Ren Miao
    • , T. Cameron Waller
    • , Don Delker
    • , Priyanka Kanth
    • , Lei Jiang
    • , Ralph J. DeBerardinis
    • , Mary P. Bronner
    • , Dean Y. Li
    • , James E. Cox
    • , Heather R. Christofk
    • , William E. Lowry
    • , Carl S. Thummel
    •  & Jared Rutter