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Letter

Nature Cell Biology 9, 1066–1073 (1 September 2007) | doi:10.1038/ncb1627

Genome-wide analysis identifies a general requirement for polarity proteins in endocytic traffic

Zita Balklava , Saumya Pant , Hanna Fares & Barth D. Grant

In a genome-wide RNA-mediated interference screen for genes required in membrane traffic — including endocytic uptake, recycling from endosomes to the plasma membrane, and secretion — we identified 168 candidate endocytosis regulators and 100 candidate secretion regulators. Many of these candidates are highly conserved among metazoans but have not been previously implicated in these processes.