Article abstract
Nature Cell Biology 9, 255 - 267 (2007)
Published online: 11 February 2007 | doi:10.1038/ncb1542
Pericytes of human skeletal muscle are myogenic precursors distinct from satellite cells
Arianna Dellavalle1,12, Maurilio Sampaolesi1,2,12, Rossana Tonlorenzi11, Enrico Tagliafico3, Benedetto Sacchetti4, Laura Perani1, Anna Innocenzi1, Beatriz G. Galvez1, Graziella Messina1,5, Roberta Morosetti6, Sheng Li7, Marzia Belicchi8, Giuseppe Peretti1, Jeffrey S. Chamberlain7, Woodring E. Wright9, Yvan Torrente8, Stefano Ferrari3, Paolo Bianco4,10 & Giulio Cossu1,4,11
Abstract
Cells derived from blood vessels of human skeletal muscle can regenerate skeletal muscle, similarly to embryonic mesoangioblasts. However, adult cells do not express endothelial markers, but instead express markers of pericytes, such as NG2 proteoglycan and alkaline phosphatase (ALP), and can be prospectively isolated from freshly dissociated ALP+ cells. Unlike canonical myogenic precursors (satellite cells), pericyte-derived cells express myogenic markers only in differentiated myotubes, which they form spontaneously with high efficiency. When transplanted into severe combined immune deficient–X-linked, mouse muscular dystrophy (scid–mdx) mice, pericyte-derived cells colonize host muscle and generate numerous fibres expressing human dystrophin. Similar cells isolated from Duchenne patients, and engineered to express human mini-dystrophin, also give rise to many dystrophin-positive fibres in vivo. These data show that myogenic precursors, distinct from satellite cells, are associated with microvascular walls in the human skeletal muscle, may represent a correlate of embryonic 'mesoangioblasts' present after birth and may be a promising candidate for future cell-therapy protocols in patients.
- Stem Cell Research Institute, San Raffaele Scientific Institute, 58 Via Olgettina, 20132 Milan, Italy.
- Department of Experimental Medicine, University of Pavia, 6 Via Forlanini, 27100 Pavia, Italy.
- Department of Biomedical Sciences, University of Modena and Reggio Emilia, 287 Via Campi, 41100 Modena, Italy.
- Institute of Cell Biology and Tissue Engineering, San Raffaele Biomedical Science Park, 100/2 Via Castel Romano, 00128 Rome, Italy.
- Department of Cellular and Developmental Biology, University of Rome La Sapienza, 5 Piazza Aldo Moro, 00161 Rome, Italy.
- Department of Neurology, Catholic University, 8 Largo A. Gemelli, 00168 Rome, Italy.
- Department of Neurology, University of Washington, 1959 N.E. Pacific Street, Seattle, WA 98195-7720. USA.
- Department of Neurological Science, Ospedale Maggiore Policlinico, University of Milan, 35 Via Francesco Sforza, 20122 Milan, Italy.
- UT Southwestern Medical Center, Dallas, 5323 Harry Hines Blvd., Dallas, TX 75390-9039, USA.
- Department of Experimental Pathology, University of Rome La Sapienza, 324 Via Regina Elena, 00161 Rome, Italy.
- Department of Biology, University of Milan, 26 Via Celoria, 20130 Milan, Italy.
- These authors contributed equally to this work.
Correspondence to: Giulio Cossu1,4,11 e-mail: cossu.giulio@hsr.it
Correspondence to: Paolo Bianco4,10 e-mail: p.bianco@flashnet.it
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