Figure 3 - Cortical DHC-1 is dependent on LIN-5, GPR-1/2 and G.

(a–h) Wild-type, lin-5(RNAi), gpr-1/2(RNAi) and G(RNAi) one-cell and two-cell stage embryos, as indicated, stained with antibodies against DHC-1 (red), -tubulin (green) and counterstained with Hoechst to view DNA (blue). Left panels show DHC-1 signal alone, right panels the merge of the three signals. (i–l) Quantification of cortical DHC-1 in wild-type (i), lin-5(RNAi) (j), gpr-1/2(RNAi) (k) and G(RNAi) (l) two-cell stage embryos. In both Fig. 3 and Fig. 4, mean line scans (s.e.m.) (95% confidence interval) are shown across rectangles 6 m wide and 5–10 m high, centred around the boundary between AB and P₁ blastomeres (see yellow rectangles in e-h; the boundary is at position 0 in the line scans; D_cortex corresponds to distance from the boundary); the relative fluorescence represents, for each embryo, values along the line scan normalized over the average of these values for the entire line-scan; n=10 embryos for each genotype, except G(RNAi) (n=8). Note increase in fluorescence intensities in cortical areas (between stippled lines) of the wild type (i), which is not apparent in lin-5(RNAi) (j), gpr-1/2(RNAi) (k) and G(RNAi) (l) embryos. Note also that quantification was conducted in two-cell stage embryos because the cortical staining is weaker and somewhat more variable in the one-cell stage; nonetheless, qualitatively analogous findings were made in one-cell stage embryos (see panels a–d and Supplementary Information, Fig. S3).