Article abstract


Nature Cell Biology 9, 1233 - 1242 (2007)
Published online: 14 October 2007 | doi:10.1038/ncb1643

Architectural dynamics of the meiotic spindle revealed by single-fluorophore imaging

Ge Yang1,4, Benjamin R. Houghtaling2,4, Jedidiah Gaetz2,3, Jenny Z. Liu2, Gaudenz Danuser1 & Tarun M. Kapoor2


Bipolarity of the meiotic spindle, required for proper chromosome segregation, is maintained throughout cell division despite rapid microtubule turnover. How this is achieved has remained mysterious, as determining the organization of individual spindle microtubules has been difficult. Here, we develop single-fluorophore speckle imaging to examine microtubule organization in the vertebrate meiotic spindle. We find that the mean length of microtubules is approx40% of spindle length. Long and short filaments distribute randomly throughout the spindle and those in close proximity can move in the same direction with highly heterogeneous velocities. The ratio between microtubule and spindle lengths remains unchanged as spindles elongate upon dynein–dynactin inhibition. However, maintaining this ratio depends on proper kinesin-5 function. Our data suggest that force transmission within the spindle must be understood in terms of the crosslinking dynamics of a tiled array of individual filaments, most of which do not span the distance from the pole to the metaphase plate.

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  1. Laboratory for Computational Cell Biology, Scripps Research Institute, 10550 North Torrey Pines Road, Mail Box CB167, La Jolla, CA 92037, USA.
  2. Laboratory of Chemistry and Cell Biology, Rockefeller University, 1230 York Avenue, Mail Box 202, New York, NY 10065, USA.
  3. Current address: Department of Human Genetics, University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA.
  4. These authors contributed equally to this work.

Correspondence to: Gaudenz Danuser1 e-mail: gdanuser@scripps.edu

Correspondence to: Tarun M. Kapoor2 e-mail: kapoor@rockefeller.edu



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