Article abstract

Nature Cell Biology 9, 45 - 56 (2006)
Published online: 17 December 2006 | doi:10.1038/ncb1516

Functional interaction between PML and SATB1 regulates chromatin-loop architecture and transcription of the MHC class I locus

Pavan Kumar P.1,2,4, Oliver Bischof2,4, Prabhat Kumar Purbey1, Dimple Notani1, Henning Urlaub3, Anne Dejean2 & Sanjeev Galande1

The function of the subnuclear structure the promyelocytic leukaemia (PML) body is unclear largely because of the functional heterogeneity of its constituents. Here, we provide the evidence for a direct link between PML, higher-order chromatin organization and gene regulation. We show that PML physically and functionally interacts with the matrix attachment region (MAR)-binding protein, special AT-rich sequence binding protein 1 (SATB1) to organize the major histocompatibility complex (MHC) class I locus into distinct higher-order chromatin-loop structures. Interferon gamma (IFNgamma) treatment and silencing of either SATB1 or PML dynamically alter chromatin architecture, thus affecting the expression profile of a subset of MHC class I genes. Our studies identify PML and SATB1 as a regulatory complex that governs transcription by orchestrating dynamic chromatin-loop architecture.

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  1. National Centre for Cell Science, Ganeshkhind, Pune 411007, India.
  2. Unité d'Organisation Nucléaire et Oncogénèse/INSERM U579, Institut Pasteur, 28, rue du Docteur Roux, 75724 Paris CEDEX 15, France.
  3. Max-Planck-Institute for Biophysical Chemistry, Bioanalytical Mass Spectrometry Group, Am Fassberg 11, D-37077 Goettingen, Germany.
  4. These authors contributed equally to this work.

Correspondence to: Sanjeev Galande1 e-mail: sanjeev@nccs.res.in



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