Brief Communication abstract
Nature Cell Biology 8, 1032 - 1034 (2006)
Published online: 6 August 2006 | doi:10.1038/ncb1466
Smc5–Smc6 mediate DNA double-strand-break repair by promoting sister-chromatid recombination
Giacomo De Piccoli1,8, Felipe Cortes-Ledesma2,8, Gregory Ira3,4,8, Jordi Torres-Rosell1,5, Stefan Uhle1, Sarah Farmer1, Ji-Young Hwang6, Felix Machin1, Audrey Ceschia1, Alexandra McAleenan1, Violeta Cordon-Preciado1, Andrés Clemente-Blanco1, Felip Vilella-Mitjana1, Pranav Ullal1, Adam Jarmuz1, Beatriz Leitao1, Debra Bressan3, Farokh Dotiwala3, Alma Papusha4, Xiaolan Zhao7, Kyungjae Myung6, James E. Haber3, Andrés Aguilera2 & Luis Aragón1
DNA double-strand breaks (DSB) can arise during DNA replication, or after exposure to DNA-damaging agents, and their correct repair is fundamental for cell survival and genomic stability. Here, we show that the Smc5–Smc6 complex is recruited to DSBs de novo to support their repair by homologous recombination between sister chromatids. In addition, we demonstrate that Smc5–Smc6 is necessary to suppress gross chromosomal rearrangements. Our findings show that the Smc5–Smc6 complex is essential for genome stability as it promotes repair of DSBs by error-free sister-chromatid recombination (SCR), thereby suppressing inappropriate non-sister recombination events.
- Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, UK.
- Departamento de Genética, Facultad de Biología, Universidad de Sevilla, 41012 Sevilla, Spain.
- Rosenstiel Center, Brandeis University, 415 South St., Mail Stop 029, Waltham, MA 02454–9110, USA.
- Baylor College of Medicine, Department of Human and Molecular Genetics, One Baylor Plaza, Houston, TX 77030, USA.
- Current address: Dept. Ciències Mèdiques Bàsiques, Facultat de Medicina, Universitat de Lleida, Montserrat Roig, 2, 25008 Lleida, Spain.
- Genome Instability Section, Genetics & Molecular Biology Branch, National Human Genome Research Institute, NIH, 49 Convent Drive, Bethesda, MD 20892–4442, USA.
- Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA.
- These authors contributed equally to this work.
Correspondence to: James E. Haber3 e-mail: haber@brandeis.edu
Correspondence to: Andrés Aguilera2 e-mail: aguilo@us.es
Correspondence to: Luis Aragón1 e-mail: luis.aragon@csc.mrc.ac.uk
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