Abstract
In the fission yeast Schizosaccharomyces pombe, cytokinesis is thought to be controlled by the daughter spindle-pole body (SPB) through a regulatory pathway named the septation initiation network (SIN). Here, we demonstrate that laser ablation of both, but not a single SPB, results in failure of cytokinesis. Ablation of only the daughter SPB often leads to activation of the SIN on the mother SPB and successful cytokinesis. Thus, either SPB can drive cytokinesis.
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Acknowledgements
This work was supported by National Institutes of Health grants GMS 59363 (to A.K.), GMS 69670 (to F.C), and by the Human Frontiers Science Program grant RGP0064 (to A.K). We thank D. McCollum and V. Simanis for strains, and R. Daga for technical assistance.
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Magidson, V., Chang, F. & Khodjakov, A. Regulation of cytokinesis by spindle-pole bodies. Nat Cell Biol 8, 891–893 (2006). https://doi.org/10.1038/ncb1449
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DOI: https://doi.org/10.1038/ncb1449
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