Letter abstract

Nature Cell Biology 8, 623 - 630 (2006)
Published online: 14 May 2006 | doi:10.1038/ncb1413

Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells

Katia Ancelin1,2, Ulrike C. Lange1, Petra Hajkova1, Robert Schneider3, Andrew J. Bannister1, Tony Kouzarides1 & M. Azim Surani1


Blimp1, a transcriptional repressor, has a crucial role in the specification of primordial germ cells (PGCs) in mice at embryonic day 7.5 (E7.5)1, 2. This SET–PR domain protein can form complexes with various chromatin modifiers in a context-dependent manner3, 4. Here, we show that Blimp1 has a novel interaction with Prmt5, an arginine-specific histone methyltransferase, which mediates symmetrical dimethylation of arginine 3 on histone H2A and/or H4 tails (H2A/H4R3me2s). Prmt5 has been shown to associate with Tudor, a component of germ plasm in Drosophila melanogaster5. Blimp1–Prmt5 colocalization results in high levels of H2A/H4 R3 methylation in PGCs at E8.5. However, at E11.5, Blimp1–Prmt5 translocates from the nucleus to the cytoplasm, resulting in the loss of H2A/H4 R3 methylation at the time of extensive epigenetic reprogramming of germ cells6. Subsequently, Dhx38, a putative target of the Blimp1–Prmt5 complex, is upregulated. Interestingly, expression of Dhx38 is also seen in pluripotent embryonic germ cells that are derived from PGCs when Blimp1 expression is lost. Our study demonstrates that Blimp1 is involved in a novel transcriptional regulatory complex in the mouse germ-cell lineage.

  1. Wellcome Trust/Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK.
  2. Present address: LBMC/UMR5161, Ecole Normale Supérieure de Lyon, 46 allée d'Italie, 69364 LYON Cedex 07, France.
  3. Max-Planck-Institute of Immunobiology, Stuebeweg 51, 79108 Freiburg, Germany.

Correspondence to: M. Azim Surani1 e-mail: as10021@mole.bio.cam.ac.uk


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