Nature Cell Biology 8, 238 - 248 (2006)
Published online: 19 February 2006; | doi:10.1038/ncb1366
GM130 and GRASP65-dependent lateral cisternal fusion allows uniform Golgi-enzyme distributionManojkumar A. Puthenveedu1, 2, Collin Bachert1, Sapna Puri1, 3, Frederick Lanni1
& Adam D. Linstedt11
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA. 2
Present address: Department of Psychiatry, UCSF, San Francisco, CA 94143, USA. 3
Present address: Department of Medicine, UCSF, San Francisco, CA 94143, USA.
Correspondence should be addressed to Adam D. Linstedt linstedt@andrew.cmu.edu The mammalian Golgi apparatus exists as stacks of cisternae that are laterally linked to form a continuous membrane ribbon, but neither the molecular requirements for, nor the purpose of, Golgi ribbon formation are known. Here, we demonstrate that ribbon formation is mediated by specific membrane-fusion events that occur during Golgi assembly, and require the Golgi proteins GM130 and GRASP65. Furthermore, these GM130 and GRASP65-dependent lateral cisternal-fusion reactions are necessary to achieve uniform distribution of enzymes in the Golgi ribbon. The membrane continuity created by ribbon formation facilitates optimal processing conditions in the biosynthetic pathway.
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