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Letter

Nature Cell Biology 8, 180–187 (1 February 2006) | doi:10.1038/ncb1350

Complex formation of Plk1 and INCENP required for metaphase|[ndash]|anaphase transition

Hidemasa Goto , Tohru Kiyono , Yasuko Tomono , Aie Kawajiri , Takeshi Urano , Koichi Furukawa , Erich A. Nigg & Masaki Inagaki

Mitotic chromosomal dynamics is regulated by the coordinated activities of many mitotic kinases, such as cyclin-dependent kinase 1 (Cdk1), Aurora-B or Polo-like kinase 1 (Plk1), but the mechanisms of their coordination remain unknown. Here, we report that Cdk1 phosphorylates Thr 59 and Thr 388 on inner centromere protein (INCENP), which regulates the localization and kinase activity of Aurora-B from prophase to metaphase.