Nature Cell Biology
- 8, 1383 - 1388 (2006)
Published online: 5 November 2006; | doi:10.1038/ncb1505
Exo70 interacts with the Arp2/3 complex and regulates cell migrationXiaofeng Zuo1, Jian Zhang1, Ying Zhang2, Shu-Chan Hsu3, Daoguo Zhou2 & Wei Guo11
Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA. 2
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA. 3
Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ 08854, USA.
Correspondence should be addressed to Wei Guo guowei@sas.upenn.edu The exocyst is a multiprotein complex essential for tethering secretory vesicles to specific domains of the plasma membrane for exocytosis1,
2,
3. Here, we report that the exocyst component Exo70 interacts with the Arp2/3 complex, a key regulator of actin polymerization4,
5. We further show that the exocyst–Arp2/3 interaction is regulated by epidermal growth factor (EGF) signalling. Inhibition of Exo70 by RNA interference (RNAi) or antibody microinjection blocks the formation of actin-based membrane protrusions and affects various aspects of cell motility. We propose that Exo70, in addition to functioning in exocytosis, also regulates actin at the leading edges of migrating cells, therefore coordinating cytoskeleton and membrane traffic during cell migration.
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