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Par6–aPKC uncouples ErbB2 induced disruption of polarized epithelial organization from proliferation control

Nature Cell Biology volume 8, pages 1235–1245 (2006)Cite this article

Abstract

The polarized glandular organization of epithelial cells is frequently lost during development of carcinoma. However, the specific oncogene targets responsible for polarity disruption have not been identified. Here, we demonstrate that activation of ErbB2 disrupts apical–basal polarity by associating with Par6–aPKC, components of the Par polarity complex. Inhibition of interaction between Par6 and aPKC blocked the ability of ErbB2 to disrupt the acinar organization of breast epithelia and to protect cells from apoptosis but was not required for cell proliferation. Therefore, oncogenes target polarity proteins to disrupt glandular organization and protect cells from apoptotic death during development of carcinoma.

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Figure 1: ErbB2 initiates disruption of apical–basal polarity at the apical–basal border.
Figure 2: ErbB2 disrupts the Par complex and recruits Par6–aPKC.
Figure 3: Par6–aPKC is required for ErbB2-induced transformation of MCF10A three-dimensional (3D) acini.
Figure 4: ErbB2–Par6–aPKC interaction is not required for ErbB2-induced proliferation.
Figure 5: Increased apoptosis in response to ErbB2 activation in cells expressing Par6K19A.
Figure 6: Bcl-2 expression partially rescues ErbB2-induced disruption of epithelial organization.
Figure 7: Loss of Scribble rescues formation of multi-acinar structures.

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Acknowledgements

We would like to thank members of the Muthuswamy laboratory for all their input and helpful discussions. We thank M. Zaratiegui for statistical analysis and interpretation and ARIAD pharmaceutical for the synthetic dimerizing ligand, AP1510. S.K.M. was supported by CA098830 from the National Cancer Institute (NCI), The V foundation Scholar award, Rita Allen Scholar award, Find a Cure Today (FACT), Glencove cares and Long Islanders Against Breast Cancer (LIBC) foundation. T.P. was supported by NCI Canada. T.H. was supported by a fellowship from the Department of Defense (DAMD17-03-1-0194). M.E.N. was also supported by a fellowship from the Department of Defense (DAMD17-03-0193). We dedicate this work to the memory of our friend and colleague, Teresa Haire.

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Author notes

  1. Victoria Aranda and Teresa Haire: These authors contributed equally to this work.

Authors and Affiliations

  1. Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, 11724, NY, USA

    Victoria Aranda, Teresa Haire, Marissa E. Nolan, Avi Z. Rosenberg & Senthil K. Muthuswamy

  2. Department of Molecular Genetics and Microbiology, Stony Brook University, 11794, NY, USA

    Teresa Haire & Senthil K. Muthuswamy

  3. Graduate Program in Genetics, Stony Brook University, 11794, NY, USA

    Marissa E. Nolan, Avi Z. Rosenberg & Senthil K. Muthuswamy

  4. Watson School of Biological Sciences, One Bungtown Road, Cold Spring Harbor, 11724, NY, USA

    Joseph P. Calarco & Senthil K. Muthuswamy

  5. Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600, University Avenue, University of Toronto, Toronto, M5G 1X5, Ontario, Canada

    James P. Fawcett & Tony Pawson

  6. Department of Chemistry, University of Toronto, 80 Saint George St., Toronto, M5S 3H6, Canada

    Joseph P. Calarco

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Aranda, V., Haire, T., Nolan, M. et al. Par6–aPKC uncouples ErbB2 induced disruption of polarized epithelial organization from proliferation control. Nat Cell Biol 8, 1235–1245 (2006). https://doi.org/10.1038/ncb1485

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