Letter abstract
Nature Cell Biology 8, 1133 - 1142 (2006)
Published online: 24 September 2006 | doi:10.1038/ncb1475
Condensin and Repo-Man–PP1 co-operate in the regulation of chromosome architecture during mitosis
Paola Vagnarelli1, Damien F. Hudson1, Susana A. Ribeiro1,2, Laura Trinkle-Mulcahy3, Jennifer M. Spence4, Fan Lai1, Christine J. Farr4, Angus I. Lamond3 & William C. Earnshaw1
Abstract
The reversible condensation of chromosomes during cell division remains a classic problem in cell biology. Condensation requires the condensin complex1 in certain experimental systems2, 3, 4, 5, 6, 7, 8, but not in many others9, 10, 11, 12, 13, 14, 15. Anaphase chromosome segregation almost always fails in condensin-depleted cells, leading to the formation of prominent chromatin bridges and cytokinesis failure4, 9, 10, 11, 12, 13, 14, 15, 16, 17. Here, live-cell analysis of chicken DT40 cells bearing a conditional knockout of condensin subunit SMC2 revealed that condensin-depleted chromosomes abruptly lose their compact architecture during anaphase and form massive chromatin bridges. The compact chromosome structure can be preserved and anaphase chromosome segregation rescued by preventing the targeting subunit Repo-Man from recruiting protein phosphatase 1 (PP1) to chromatin at anaphase onset. This study identifies an activity critical for mitotic chromosome structure that is inactivated by Repo-Man–PP1 during anaphase. This activity, provisionally termed 'regulator of chromosome architecture' (RCA), cooperates with condensin to preserve the characteristic chromosome architecture during mitosis.
- Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Swann Building, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, UK.
- IPATIMUP, R. Roberto Frias, s/n, 4050-465 Porto, Portugal.
- MSI/WTB Complex, University of Dundee, Dow Street, Dundee DD1 5EH, UK.
- Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.
Correspondence to: William C. Earnshaw1 e-mail: Bill.Earnshaw@ed.ac.uk
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.
NEWS AND VIEWS
Cdc14 phosphatase resolves the rDNA segregation delayNature Cell Biology News and Views (01 Jun 2004)
RESEARCH
Lte1, Cdc14 and MEN-controlled Cdk inactivation in yeast coordinate rDNA decompaction with late telophase progressionThe EMBO Journal Article (03 Jun 2009)
CENP-V is required for centromere organization, chromosome alignment and cytokinesisThe EMBO Journal Article (08 Oct 2008)
Maximal chromosome compaction occurs by axial shortening in anaphase and depends on Aurora kinaseNature Cell Biology Letter (01 Jul 2007)
See all 41 matches for Research
