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Letter

Nature Cell Biology 8, 84–90 (1 January 2006) | doi:10.1038/ncb1346

PCNA functions as a molecular platform to trigger Cdt1 destruction and prevent re-replication

Emily E. Arias & Johannes C. Walter

Ubiquitin-mediated proteolysis of the replication licensing factor Cdt1 (Cdc10-dependent transcript 1) in S phase is a key mechanism that limits DNA replication to a single round per cell cycle in metazoans. In Xenopus egg extracts, Cdt1 is destroyed on chromatin during DNA replication.