Brief Communication abstract
Nature Cell Biology 7, 921 - 923 (2005)
Published online: 1 September 2005 | doi:10.1038/ncb1296
Distinct roles of I
B proteins in regulating constitutive NF-B activity
Vinay Tergaonkar1,2, Ricardo G Correa1, Masahito Ikawa3 & Inder M Verma1
The inhibitor of NF-
B (IB) family of proteins is believed to regulate NF-B activity by cytoplasmic sequestration. We show that in cells depleted of IB
, IB
and IB
proteins, a small fraction of p65 binds DNA and leads to constitutive activation of NF-B target genes, even without stimulation, whereas most of the p65 remains cytoplasmic. These results indicate that although IB, IB and IB proteins could be dispensable for cytoplasmic retention of NF-B, they are essential for preventing NF-B-dependent gene expression in the basal state. We also show that in the absence of IB, IB and IB proteins, cytoplasmic retention of NF-B by other cellular proteins renders the pathway unresponsive to activation.
- Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
- Present address: Institute of Molecular and Cell Biology, 61 Biopolis Drive, 138673, Singapore.
- Present address: Genome information Research Center, Osaka University, 3-1, Osaka 565-0871, Japan.
Correspondence to: Inder M Verma1 e-mail: verma@salk.edu
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