Nature Cell Biology
7, 921 - 923 (2005)
Published online: 1 September 2005; | doi:10.1038/ncb1296
Distinct roles of I B proteins in regulating constitutive NF-B activityVinay Tergaonkar1, 2, Ricardo G Correa1, Masahito Ikawa3
& Inder M Verma11
Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA. 2
Present address: Institute of Molecular and Cell Biology, 61 Biopolis Drive, 138673, Singapore. 3
Present address: Genome information Research Center, Osaka University, 3-1, Osaka 565-0871, Japan.
Correspondence should be addressed to Inder M Verma verma@salk.edu The inhibitor of NF- B (IB) family of proteins is believed to regulate NF-B activity by cytoplasmic sequestration. We show that in cells depleted of IB , IB and IB proteins, a small fraction of p65 binds DNA and leads to constitutive activation of NF-B target genes, even without stimulation, whereas most of the p65 remains cytoplasmic. These results indicate that although IB, IB and IB proteins could be dispensable for cytoplasmic retention of NF-B, they are essential for preventing NF-B-dependent gene expression in the basal state. We also show that in the absence of IB, IB and IB proteins, cytoplasmic retention of NF-B by other cellular proteins renders the pathway unresponsive to activation.
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