Nature Cell Biology7, 581 - 590 (2005)
Published online: 15 May 2005; | doi:10.1038/ncb1262
Microtubule-induced focal adhesion disassembly is mediated by dynamin and focal adhesion kinase
Ellen J. Ezratty1, Michael A. Partridge2
& Gregg G. Gundersen1, 2
1
Department of Anatomy and Cell Biology, Columbia University, 630 West 168th Street, BB 1217, New York, NY 10032, USA.
2
Department of Pathology, Columbia University, 630 West 168th Street, BB 1217, New York, NY 10032, USA.
Correspondence should be addressed to Gregg G. Gundersen ggg1@columbia.edu
Imaging studies implicate microtubule targeting of focal adhesions in focal adhesion disassembly, although the molecular mechanism is unknown. Here, we develop a model system of focal adhesion disassembly based on the finding that microtubule regrowth after nocodazole washout induces disassembly of focal adhesions, and that this disassembly occurs independently of Rho and Rac, but depends on focal adhesion kinase (FAK) and dynamin. During disassembly, dynamin interacts with FAK and colocalizes with focal adhesions. Inhibition of dynamin prevents migration of cells with a focal adhesion phenotype. Our results show that focal adhesion disassembly involves microtubules, dynamin and FAK, and is not simply the reversal of focal adhesion formation.
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.
