Policy update - p203

doi:10.1038/ncb0305-203

Full Text - Policy update | PDF (107 KB) - Policy update

Pars and polarity: taking control of Rac - pp205 - 207

Toby W. Hurd & Ben Margolis

doi:10.1038/ncb0305-205

In this issue, two groups have examined the role of the Par proteins in the regulation of small G proteins, in two different models of cell polarity — mammalian epithelia and neuronal axon specification. They concurrently report the intriguing finding that the Par-3/Par-6/aPKC complex can regulate the activation of Rac by interaction with the guanine nucleotide exchanger, Tiam1/STEF. Furthermore, they also demonstrate a hitherto under-appreciated role for the Par-3/Par-6/aPKC complex in the regulation of the actin cytoskeleton.

Full Text - Pars and polarity: taking control of Rac | PDF (169 KB) - Pars and polarity: taking control of Rac

See also: Letter by Chen & Macara | Letter by Nishimura et al.

Cdc48–Ufd2–Rad23: the road less ubiquitinated? - pp207 - 209

Omar A. Bazirgan & Randolph Y. Hampton

doi:10.1038/ncb0305-207

Targeting substrates to the proteasome is generally thought to depend on long polyubiquitin chains that form on the substrate. Recent work has led to the provocative proposal that some substrates may be targeted to the proteasome through smaller chains that contain only four to six ubiquitin molecules.

Full Text - Cdc48–Ufd2–Rad23: the road less ubiquitinated? | PDF (281 KB) - Cdc48–Ufd2–Rad23: the road less ubiquitinated?

An endocytic Prk-ing brake - pp210 - 212

Mara C. Duncan & Gregory S. Payne

doi:10.1038/ncb0305-210

Actin polymerization is intimately associated with endocytosis in yeast and mammalian cells. Mechanisms of actin filament assembly in endocytosis are becoming better understood but less is known about how assembly is terminated. In yeast, phosphorylation of Arp2/3 complex activators provides one mechanism for downregulating endocytic actin assembly.

Full Text - An endocytic Prk-ing brake | PDF (226 KB) - An endocytic Prk-ing brake

See also: Letter by Toshima et al.

Growth factor control of autophagy - p212

Markus Wagner

doi:10.1038/ncb0305-212

Full Text - Growth factor control of autophagy | PDF (127 KB) - Growth factor control of autophagy

Stress and death: breaking up the c-Abl/14-3-3 complex in apoptosis - pp213 - 214

Ann Marie Pendergast

doi:10.1038/ncb0305-213

Nuclear accumulation of the c-Abl tyrosine kinase is associated with a pro-apoptotic response after DNA damage, but the mechanism regulating c-Abl nuclear localization is unclear. DNA damage and other stress signals are now shown to induce phosphorylation of 14-3-3 proteins by the c-Jun amino-terminal kinase, disrupting a c-Abl/14-3-3 cytoplasmic complex, and liberating c-Abl for translocation to the nucleus.

Full Text - Stress and death: breaking up the c-Abl/14-3-3 complex in apoptosis | PDF (166 KB) - Stress and death: breaking up the c-Abl/14-3-3 complex in apoptosis

See also: Letter by Yoshida et al.

The Myc trilogy: lord of RNA polymerases - pp215 - 217

Thordur Oskarsson & Andreas Trumpp

doi:10.1038/ncb0305-215

Cancer cells have a raised cellular metabolism, including increased protein biosynthesis. Three new studies now show that the oncoprotein Myc, known to drive cell division, also enhances ribosomal RNA synthesis by RNA polymerase I in addition to controlling RNA polymerase II- and III-regulated gene transcription. This suggests that Myc promotes the generation of crucial components of a functional ribosome.

Full Text - The Myc trilogy: lord of RNA polymerases | PDF (237 KB) - The Myc trilogy: lord of RNA polymerases

See also: Letter by Grewal et al. | Letter by Grandori et al. | Letter by Arabi et al.

Nucleolar targeting runs on GTP cycles - p217

Sowmya Swaminathan

doi:10.1038/ncb0305-217

Full Text - Nucleolar targeting runs on GTP cycles | PDF (125 KB) - Nucleolar targeting runs on GTP cycles

Spindle assembly: asters part their separate ways - pp219 - 222

Jody Rosenblatt

doi:10.1038/ncb0305-219

Abstract - | Full Text - Spindle assembly: asters part their separate ways | PDF (535 KB) - Spindle assembly: asters part their separate ways

A new tome for the centrosome - p223

Kevin O'Connell reviews Centrosomes in Development and Disease by Erich Nigg

doi:10.1038/ncb0305-223

Full Text - A new tome for the centrosome | PDF (111 KB) - A new tome for the centrosome

Maintenance of the diacylglycerol level in the Golgi apparatus by the Nir2 protein is critical for Golgi secretory function - pp225 - 234

Vladimir Litvak, Nili Dahan, Sreekumar Ramachandran, Helena Sabanay & Sima Lev

doi:10.1038/ncb1221

Abstract - | Full Text - Maintenance of the diacylglycerol level in the Golgi apparatus by the Nir2 protein is critical for Golgi secretory function | PDF (969 KB) - Maintenance of the diacylglycerol level in the Golgi apparatus by the Nir2 protein is critical for Golgi secretory function | Supplementary information

Functionally distinct kinesin-13 family members cooperate to regulate microtubule dynamics during interphase - pp235 - 245

Vito Mennella, Gregory C. Rogers, Stephen L. Rogers, Daniel W. Buster, Ronald D. Vale & David J. Sharp

doi:10.1038/ncb1222

Abstract - | Full Text - Functionally distinct kinesin-13 family members cooperate to regulate microtubule dynamics during interphase | PDF (2,169 KB) - Functionally distinct kinesin-13 family members cooperate to regulate microtubule dynamics during interphase | Supplementary information

Phosphoregulation of Arp2/3-dependent actin assembly during receptor-mediated endocytosis - pp246 - 254

Jiro Toshima, Junko Y. Toshima, Adam C. Martin & David G. Drubin

doi:10.1038/ncb1229

First Paragraph - | Full Text - Phosphoregulation of Arp2/3-dependent actin assembly during receptor-mediated endocytosis | PDF (2,222 KB) - Phosphoregulation of Arp2/3-dependent actin assembly during receptor-mediated endocytosis | Supplementary information

See also: News and Views by Duncan & Payne

Cdc42–MRCK and Rho–ROCK signalling cooperate in myosin phosphorylation and cell invasion - pp255 - 261

Simon Wilkinson, Hugh F. Paterson & Christopher J. Marshall

doi:10.1038/ncb1230

First Paragraph - | Full Text - Cdc42–MRCK and Rho–ROCK signalling cooperate in myosin phosphorylation and cell invasion | PDF (633 KB) - Cdc42–MRCK and Rho–ROCK signalling cooperate in myosin phosphorylation and cell invasion | Supplementary information

Par-3 controls tight junction assembly through the Rac exchange factor Tiam1 - pp262 - 269

Xinyu Chen & Ian G. Macara

doi:10.1038/ncb1226

First Paragraph - | Full Text - Par-3 controls tight junction assembly through the Rac exchange factor Tiam1 | PDF (1,961 KB) - Par-3 controls tight junction assembly through the Rac exchange factor Tiam1 | Supplementary information

See also: News and Views by Hurd & Margolis

PAR-6–PAR-3 mediates Cdc42-induced Rac activation through the Rac GEFs STEF/Tiam1 - pp270 - 277

Takashi Nishimura, Tomoya Yamaguchi, Katsuhiro Kato, Masato Yoshizawa, Yo-ichi Nabeshima, Shigeo Ohno, Mikio Hoshino & Kozo Kaibuchi

doi:10.1038/ncb1227

First Paragraph - | Full Text - PAR-6–PAR-3 mediates Cdc42-induced Rac activation through the Rac GEFs STEF/Tiam1 | PDF (2,028 KB) - PAR-6–PAR-3 mediates Cdc42-induced Rac activation through the Rac GEFs STEF/Tiam1 | Supplementary information

See also: News and Views by Hurd & Margolis

JNK phosphorylation of 14-3-3 proteins regulates nuclear targeting of c-Abl in the apoptotic response to DNA damage - pp278 - 285

Kiyotsugu Yoshida, Tomoko Yamaguchi, Tohru Natsume, Donald Kufe & Yoshio Miki

doi:10.1038/ncb1228

First Paragraph - | Full Text - JNK phosphorylation of 14-3-3 proteins regulates nuclear targeting of c-Abl in the apoptotic response to DNA damage | PDF (536 KB) - JNK phosphorylation of 14-3-3 proteins regulates nuclear targeting of c-Abl in the apoptotic response to DNA damage | Supplementary information

See also: News and Views by Pendergast

Atrophy of S6K1^-/- skeletal muscle cells reveals distinct mTOR effectors for cell cycle and size control - pp286 - 294

Mickaël Ohanna, Andrew K. Sobering, Thomas Lapointe, Lazaro Lorenzo, Christophe Praud, Emmanuel Petroulakis, Nahum Sonenberg, Paul A. Kelly, Athanassia Sotiropoulos & Mario Pende

doi:10.1038/ncb1231

First Paragraph - | Full Text - Atrophy of S6K1-/- skeletal muscle cells reveals distinct mTOR effectors for cell cycle and size control | PDF (960 KB) - Atrophy of S6K1-/- skeletal muscle cells reveals distinct mTOR effectors for cell cycle and size control | Supplementary information

Myc-dependent regulation of ribosomal RNA synthesis during Drosophila development - pp295 - 302

Savraj S. Grewal, Ling Li, Amir Orian, Robert N. Eisenman & Bruce A. Edgar

doi:10.1038/ncb1223

First Paragraph - | Full Text - Myc-dependent regulation of ribosomal RNA synthesis during Drosophila development | PDF (1,585 KB) - Myc-dependent regulation of ribosomal RNA synthesis during Drosophila development | Supplementary information

See also: News and Views by Oskarsson & Trumpp

c-Myc associates with ribosomal DNA and activates RNA polymerase I transcription - pp303 - 310

Azadeh Arabi, Siqin Wu, Karin Ridderstråle, Holger Bierhoff, Chiounan Shiue, Karoly Fatyol, Sara Fahlén, Per Hydbring, Ola Söderberg, Ingrid Grummt, Lars-Gunnar Larsson & Anthony P. H. Wright

doi:10.1038/ncb1225

First Paragraph - | Full Text - c-Myc associates with ribosomal DNA and activates RNA polymerase I transcription | PDF (609 KB) - c-Myc associates with ribosomal DNA and activates RNA polymerase I transcription

See also: News and Views by Oskarsson & Trumpp

c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I - pp311 - 318

Carla Grandori, Natividad Gomez-Roman, Zoe A. Felton-Edkins, Celine Ngouenet, Denise A. Galloway, Robert N. Eisenman & Robert J. White

doi:10.1038/ncb1224

First Paragraph - | Full Text - c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I | PDF (961 KB) - c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I | Supplementary information

See also: News and Views by Oskarsson & Trumpp

Imaging of single light-responsive clock cells reveals fluctuating free-running periods - pp319 - 321

Amanda-Jayne F. Carr & David Whitmore

doi:10.1038/ncb1232

Abstract - | Full Text - Imaging of single light-responsive clock cells reveals fluctuating free-running periods | PDF (549 KB) - Imaging of single light-responsive clock cells reveals fluctuating free-running periods | Supplementary information