Nature Cell Biology 7, 1240 - 1247 (2005)
Published online: 6 November 2005; | doi:10.1038/ncb1335
There is an Erratum (December 2005) associated with this Letter.
RNA-dependent integrin  3 protein localization regulated by the Muscleblind-like protein MLP1Yair Adereth, Vincent Dammai, Nurgun Kose, Runzhao Li
& Tien Hsu
Department of Pathology and Laboratory Medicine, and Hollings Cancer Center, Medical University of South Carolina, 86 Jonathan Lucas St, Room 330, Charleston, SC 29425, USA
Correspondence should be addressed to Tien Hsu hsut@musc.edu We show that localized expression of the integrin  3 protein is regulated at the level of RNA localization by the human homologue of Drosophila Muscleblind, MLP1/MBLL/MBNL2, a unique Cys3His zinc-finger protein. This is supported by the following observations: MLP1 knockdown abolishes localization of integrin 3 to the adhesion complexes; MLP1 is localized in adhesion plaques that contain phospho-focal adhesion kinase; this localization is microtubule-dependent; integrin 3 transcripts colocalize with MLP1 in distinct cytoplasmic loci; integrin 3 transcripts are physically associated with MLP1 in cells and MLP1 binds to a specific ACACCC motif in the integrin 3 3' untranslated region (UTR) in vitro; and a green fluorescent protein (GFP) open reading frame–integrin 3 3' UTR chimeric gene directs GFP protein localization to distinct cytoplasmic loci near the cell periphery, which is dependent on MLP1 and is mediated by the ACACCC motif but is independent of the integrin 3 signal peptide.
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