Article abstract
Nature Cell Biology 7, 1179 - 1190 (2005)
Published online: 20 November 2005 | doi:10.1038/ncb1325
There is an Erratum (December 2005) associated with this Article.
mPins modulates PSD-95 and SAP102 trafficking and influences NMDA receptor surface expression
Nathalie Sans1,4, Philip Y. Wang1, Quansheng Du2, Ronald S. Petralia1, Ya-Xian Wang1, Sajan Nakka1, Joe B. Blumer3, Ian G. Macara2 & Robert J. Wenthold1
Abstract
Appropriate trafficking and targeting of glutamate receptors (GluRs) to the postsynaptic density is crucial for synaptic function. We show that mPins (mammalian homologue of Drosophila melanogaster partner of inscuteable) interacts with SAP102 and PSD-95 (two PDZ proteins present in neurons), and functions in the formation of the NMDAR–MAGUK (N-methyl-D-aspartate receptor–membrane-associated guanylate kinase) complex. mPins enhances trafficking of SAP102 and NMDARs to the plasma membrane in neurons. Expression of dominant–negative constructs and short-interfering RNA (siRNA)-mediated knockdown of mPins decreases SAP102 in dendrites and modifies surface expression of NMDARs. mPins changes the number and morphology of dendritic spines and these effects depend on its G
i interaction domain, thus implicating G-protein signalling in the regulation of postsynaptic structure and trafficking of GluRs.
- Laboratory of Neurochemistry, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Building 50, Room 4146, 50 South Drive, Bethesda, MD 20892-8027, USA.
- Center for Cell Signaling and Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA.
- Department of Pharmacology and Experimental Therapeutics, LSU Health Sciences Center, 1901 Perdido St., P7-1, New Orleans, LA 70112, USA.
- Present address: Equipe AVENIR, IFR8, Institut National de la Sante et de la Recherche Medicale, Institut des Neurosciences F. Magendie, 33077 Bordeaux Cedex, France.
Correspondence to: Nathalie Sans1,4 e-mail: nathalie.sans@bordeaux.inserm.fr
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