Abstract
Actin polymerization is a key process for many cellular events during development. To a large extent, the formation of filamentous actin is controlled by the WASP and WAVE proteins that activate the Arp2/3 complex in different developmental processes1,2,3. WAVE function is regulated through a protein complex containing Sra1, Kette and Abi. Using biochemical, cell biological and genetic tools, we show here that the Abi protein also has a central role in activating WASP-mediated processes. Abi binds WASP through its carboxy-terminal domain and acts as a potent stimulator of WASP-dependent F-actin formation. To elucidate the biological function of abi in Drosophila melanogaster, we studied bristle development, a process known to require wasp function4. Reduction of abi function leads to a loss of bristles similar to that observed in wasp mutants. Activation of Abi results in the formation of ectopic bristles, a phenotype that is suppressed by a reduction of wasp activity but is not affected by the reduction of wave function. Thus, in vivo Abi may set the balance between WASP and WAVE in different actin-based developmental processes.
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Acknowledgements
We thank E. Schejter for generously sending materials (scark13811, scarΔ37, wasp3, wasp1 mutants and UAS-waspΔCA flies), J. Zallen and G. Borisy for sending anti-WAVE and anti-WASP antibodies, G. Scita for sharing and discussing results prior to publication, S. Wenderdel for excellent technical assistance, S. Call for purified His-tagged WAVE protein, A. Püschel, V. Gerke and T. Hummel for helpful discussions and comments on the manuscript, and members of the Klämbt laboratory for help throughout the project. This work was funded through a grant of the DFG and the EC (MAIN initiative).
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Bogdan, S., Stephan, R., Löbke, C. et al. Abi activates WASP to promote sensory organ development. Nat Cell Biol 7, 977–984 (2005). https://doi.org/10.1038/ncb1305
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DOI: https://doi.org/10.1038/ncb1305
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