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Letter

Nature Cell Biology 7, 1014–1020 (1 October 2005) | doi:10.1038/ncb1301

Neddylation and deneddylation regulate Cul1 and Cul3 protein accumulation

June-Tai Wu , Hsiu-Chen Lin , Yen-Chen Hu & Cheng-Ting Chien

Cullin family proteins organize ubiquitin ligase (E3) complexes to target numerous cellular proteins for proteasomal degradation. Neddylation, the process that conjugates the ubiquitin-like polypeptide Nedd8 to the conserved lysines of cullins, is essential for in vivo cullin-organized E3 activities.