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Nature Cell Biology 7, 30–41 (1 January 2005) | doi:10.1038/ncb1202

Akt and 14-3-3|[eta]| regulate Miz1 to control cell-cycle arrest after DNA damage

Michael Wanzel , Daniela Kleine-Kohlbrecher , Steffi Herold , Andreas Hock , Katrien Berns , Jongsun Park , Brian Hemmings & Martin Eilers

The transcription factor Miz1 is required for DNA-damage-induced cell-cycle arrest. We have now identified 14-3-3|[eta]| as a gene that inhibits Miz1 function through interaction with its DNA binding domain. Binding of 14-3-3|[eta]| to Miz1 depends on phosphorylation by Akt and regulates the recovery of cells from arrest after DNA damage. Miz1 has two functions in response to DNA damage: first, it is required for upregulation of a large group of genes, a function that is regulated by c-Myc, but not by 14-3-3|[eta]|; second, Miz1 represses the expression of many genes in response to DNA damage in an Akt- and 14-3-3|[eta]|-regulated manner.