Letter abstract
Nature Cell Biology 7, 56 - 62 (2004)
Published online: 5 December 2004 | doi:10.1038/ncb1203
A critical role for Cyclin E in cell fate determination in the central nervous system of Drosophila melanogaster
Christian Berger1, S. K. Pallavi2, Mohit Prasad2, L. S. Shashidhara2 & Gerhard M. Technau1
We have examined the process by which cell diversity is generated in neuroblast (NB) lineages in the central nervous system of Drosophila melanogaster. Thoracic NB6-4 (NB6-4t) generates both neurons and glial cells, whereas NB6-4a generates only glial cells in abdominal segments1. This is attributed to an asymmetric first division of NB6-4t, localizing prospero (pros) and glial cell missing (gcm) only to the glial precursor cell, and a symmetric division of NB6-4a, where both daughter cells express pros and gcm2, 3, 4, 5. Here we show that the NB6-4t lineage represents the ground state, which does not require the input of any homeotic gene, whereas the NB6-4a lineage is specified by the homeotic genes abd-A and Abd-B. They specify the NB6-4a lineage by down-regulating levels of the G1 cyclin, DmCycE (CycE). CycE, which is asymmetrically expressed after the first division of NB6-4t, functions upstream of pros and gcm to specify the neuronal sublineage. Loss of CycE function causes homeotic transformation of NB6-4t to NB6-4a, whereas ectopic CycE induces reverse transformations. However, other components of the cell cycle seem to have a minor role in this process, suggesting a critical role for CycE in regulating cell fate in segment-specific neural lineages.
- Institute for Genetics, University of Mainz, D-55099 Mainz, Germany.
- Center for Cellular and Molecular Biology, Uppal Road, 500 007 Hyderabad, India.
Correspondence to: L. S. Shashidhara2 e-mail: shashi@ccmb.res.in
Correspondence to: Gerhard M. Technau1 e-mail: technau@uni-mainz.de
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