Sliding across a cytoskeletal milestone - p375

doi:10.1038/ncb0504-375

Full Text - Sliding across a cytoskeletal milestone | PDF (59 KB) - Sliding across a cytoskeletal milestone

The Promethean Fire of Cloning - p377

R. Alta Charo reviews A Clone of Your Own? The science and ethics of cloning by Arlene Judith Klotzko

doi:10.1038/ncb0504-377

Full Text - The Promethean Fire of Cloning | PDF (77 KB) - The Promethean Fire of Cloning

N-terminal acetylation targets GTPases to membranes - pp379 - 380

Catherine L. Jackson

doi:10.1038/ncb0504-379

The small GTPases Arl3p and Arl1p function sequentially to recruit diverse effector proteins to the Golgi apparatus. Similarly to ARF proteins, Arl1p is targeted to membranes by myristoylation. Arl3p, however, is not myristoylated. Recent work demonstrates that Arl3p, and its mammalian orthologue ARFRP1, are targeted to membranes by amino-terminal acetylation, which facilitates recognition by the membrane receptor Sys1p/hSys1.

Full Text - N-terminal acetylation targets GTPases to membranes | PDF (149 KB) - N-terminal acetylation targets GTPases to membranes

See also: Article by Behnia et al. | Article by Setty et al.

Egalitarian and the case of the missing link - pp381 - 383

John Pearson & Acaimo González-Reyes

doi:10.1038/ncb0504-381

The specificity of cytoskeleton-mediated cargo transport is critical for cell polarization. The Egalitarian protein — required for the localization of mRNAs and proteins to the oocyte of the Drosophila melanogaster ovary — is now reported to interact with an integral component of the dynein motor complex, the dynein light chain Dlc1.

Full Text - Egalitarian and the case of the missing link | PDF (205 KB) - Egalitarian and the case of the missing link

See also: Letter by Navarro et al.

Human disease: the centrosome connection - p383

Sowmya Swaminathan

doi:10.1038/ncb0504-383

Full Text - Human disease: the centrosome connection | PDF (144 KB) - Human disease: the centrosome connection

New candidates for vesicle coat proteins - pp384 - 385

Michael Roth

doi:10.1038/ncb0504-384

Proteins exit the trans-Golgi network (TGN) through multiple mechanisms that are poorly understood. New work identifies FAPP1 and FAPP2 as TGN-localized proteins that regulate post-Golgi carrier formation through interactions with phosphatidylinositol 4-phosphate (PtdIns(4)P) and ARF1.

Full Text - New candidates for vesicle coat proteins | PDF (112 KB) - New candidates for vesicle coat proteins

See also: Article by Godi et al.

Fatal liaisons of p53 with Bax and Bak - pp386 - 388

Jean-Luc Perfettini, Romano T. Kroemer & Guido Kroemer

doi:10.1038/ncb0504-386

p53 can induce apoptosis through its well defined role as a regulator of transcription, but also by a less well defined transcription-independent mechanism. Two recent studies demonstrate that p53 mediates mitochondrial permeabilization through direct physical interactions with the pro-apoptotic Bcl-2 family members, Bax and Bak.

Full Text - Fatal liaisons of p53 with Bax and Bak | PDF (256 KB) - Fatal liaisons of p53 with Bax and Bak

See also: Letter by Leu et al.

A Bit-role for integrins in apoptosis - pp388 - 389

Dwayne G. Stupack & David A. Cheresh

doi:10.1038/ncb0504-388

Loss of integrin-mediated attachment to the extracellular matrix triggers apoptosis through the activation of caspases. A recent study shows that disruption of integrin function also induces mitochondrial release of Bit1, a caspase-independent effector of apoptosis.

Full Text - A Bit-role for integrins in apoptosis | PDF (113 KB) - A Bit-role for integrins in apoptosis

Actin dynamics: re-drawing the map - pp390 - 391

Phillip R. Gordon-Weeks

doi:10.1038/ncb0504-390

The microtubule-associated proteins MAP2 and tau are important for regulating microtubule dynamics and bundling, but can also bind to actin filaments. It seems, however, that only MAP2 can bind directly to actin filaments and, curiously, the actin-binding domain is situated within the microtubule-binding domain.

Full Text - Actin dynamics: re-drawing the map | PDF (116 KB) - Actin dynamics: re-drawing the map

Cell of the month: Differentiating mouse embryonic stem cells - p392

Lia Scotti Campos

doi:10.1038/ncb0504-392

Full Text - Cell of the month: Differentiating mouse embryonic stem cells | PDF (429 KB) - Cell of the month: Differentiating mouse embryonic stem cells

FAPPs control Golgi-to-cell-surface membrane traffic by binding to ARF and PtdIns(4)P - pp393 - 404

Anna Godi, Antonella Di Campli, Athanasios Konstantakopoulos, Giuseppe Di Tullio, Dario R. Alessi, Gursant S. Kular, Tiziana Daniele, Pierfrancesco Marra, John M. Lucocq & M. Antonietta De Matteis

doi:10.1038/ncb1119

Abstract - | Full Text - FAPPs control Golgi-to-cell-surface membrane traffic by binding to ARF and PtdIns(4)P | PDF (1,788 KB) - FAPPs control Golgi-to-cell-surface membrane traffic by binding to ARF and PtdIns(4)P | Supplementary information

See also: News and Views by Roth

Targeting of the Arf-like GTPase Arl3p to the Golgi requires N-terminal acetylation and the membrane protein Sys1p - pp405 - 413

Rudy Behnia, Bojana Panic, James R. C. Whyte & Sean Munro

doi:10.1038/ncb1120

Abstract - | Full Text - Targeting of the Arf-like GTPase Arl3p to the Golgi requires N-terminal acetylation and the membrane protein Sys1p | PDF (2,050 KB) - Targeting of the Arf-like GTPase Arl3p to the Golgi requires N-terminal acetylation and the membrane protein Sys1p | Supplementary information

See also: News and Views by Jackson

Golgi targeting of ARF-like GTPase Arl3p requires its N-acetylation and the integral membrane protein Sys1p - pp414 - 419

Subba Rao Gangi Setty, Todd I. Strochlic, Amy Hin Yan Tong, Charles Boone & Christopher G. Burd

doi:10.1038/ncb1121

Abstract - | Full Text - Golgi targeting of ARF-like GTPase Arl3p requires its N-acetylation and the integral membrane protein Sys1p | PDF (4,122 KB) - Golgi targeting of ARF-like GTPase Arl3p requires its N-acetylation and the integral membrane protein Sys1p | Supplementary information

See also: News and Views by Jackson

PtdIns(3,4,5)P₃ binding is necessary for WAVE2-induced formation of lamellipodia - pp420 - 426

Tsukasa Oikawa, Hideki Yamaguchi, Toshiki Itoh, Masayoshi Kato, Takeshi Ijuin, Daisuke Yamazaki, Shiro Suetsugu & Tadaomi Takenawa

doi:10.1038/ncb1125

First Paragraph - | Full Text - PtdIns(3,4,5)P3 binding is necessary for WAVE2-induced formation of lamellipodia | PDF (1,587 KB) - PtdIns(3,4,5)P3 binding is necessary for WAVE2-induced formation of lamellipodia | Supplementary information

Egalitarian binds dynein light chain to establish oocyte polarity and maintain oocyte fate - pp427 - 435

Caryn Navarro, Hamsa Puthalakath, Jerry M. Adams, Andreas Strasser & Ruth Lehmann

doi:10.1038/ncb1122

First Paragraph - | Full Text - Egalitarian binds dynein light chain to establish oocyte polarity and maintain oocyte fate | PDF (1,721 KB) - Egalitarian binds dynein light chain to establish oocyte polarity and maintain oocyte fate | Supplementary information

See also: News and Views by Pearson & González-Reyes

In vivo self-renewing divisions of haematopoietic stem cells are increased in the absence of the early G1-phase inhibitor, p18^INK4C - pp436 - 442

Youzhong Yuan, Hongmei Shen, David S. Franklin, David T. Scadden & Tao Cheng

doi:10.1038/ncb1126

First Paragraph - | Full Text - In vivo self-renewing divisions of haematopoietic stem cells are increased in the absence of the early G1-phase inhibitor, p18INK4C | PDF (258 KB) - In vivo self-renewing divisions of haematopoietic stem cells are increased in the absence of the early G1-phase inhibitor, p18INK4C | Supplementary information

Mitochondrial p53 activates Bak and causes disruption of a Bak–Mcl1 complex - pp443 - 450

J. I-Ju Leu, Patrick Dumont, Michael Hafey, Maureen E. Murphy & Donna L. George

doi:10.1038/ncb1123

First Paragraph - | Full Text - Mitochondrial p53 activates Bak and causes disruption of a Bak–Mcl1 complex | PDF (205 KB) - Mitochondrial p53 activates Bak and causes disruption of a Bak–Mcl1 complex

See also: News and Views by Perfettini et al.

Amplitude control of cell-cycle waves by nuclear import - pp451 - 457

Attila Becskei, Monica G. Boselli & Alexander van Oudenaarden

doi:10.1038/ncb1124

First Paragraph - | Full Text - Amplitude control of cell-cycle waves by nuclear import | PDF (1,048 KB) - Amplitude control of cell-cycle waves by nuclear import | Supplementary information

An epididymis-specific -defensin is important for the initiation of sperm maturation - pp458 - 464

Chen Xi Zhou, Yong-Lian Zhang, Liqing Xiao, Min Zheng, Ka Man Leung, Man Yee Chan, Pui Shan Lo, Lai Ling Tsang, Hau Yan Wong, Lok Sze Ho, Yiu Wa Chung & Hsiao Chang Chan

doi:10.1038/ncb1127

First Paragraph - | Full Text - An epididymis-specific -defensin is important for the initiation of sperm maturation | PDF (398 KB) - An epididymis-specific -defensin is important for the initiation of sperm maturation

Errata: Histone H3 lysine 4 methylation patterns in higher eukaryotic genes - p465

doi:10.1038/ncb1128

Full Text - Errata: Histone H3 lysine 4 methylation patterns in higher eukaryotic genes | PDF (47 KB) - Errata: Histone H3 lysine 4 methylation patterns in higher eukaryotic genes

Erratum: A physical and functional map of the human TNF-/NF-B signal transduction pathway - p465

doi:10.1038/ncb1129

Full Text - Erratum: A physical and functional map of the human TNF-/NF-B signal transduction pathway | PDF (32 KB) - Erratum: A physical and functional map of the human TNF-/NF-B signal transduction pathway

Erratum: Book Review: The Golgi in plants and animals - p465

doi:10.1038/ncb1130

Full Text - Erratum: Book Review: The Golgi in plants and animals | PDF (11 KB) - Erratum: Book Review: The Golgi in plants and animals