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Cytoplasmic ubiquitin ligase KPC regulates proteolysis of p27Kip1 at G1 phase

Takumi Kamura, Taichi Hara, Masaki Matsumoto, Noriko Ishida, Fumihiko Okumura, Shigetsugu Hatakeyama, Minoru Yoshida, Keiko Nakayama & Keiichi I. Nakayama

Nature Cell Biology 6, 1229 - 1235 (2004) Published online: 7 November 2004

doi:10.1038/ncb1194

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Figure 1

Purification of KPC.

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Figure 2

Polyubiquitination of p27Kip1 by KPC.

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Figure 3

Interaction between KPC and p27Kip1 and subcellular localization of KPC.

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Figure 4

Effect of KPC overexpression or depletion on p27Kip1 degradation.

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Figure 5

Effects of KPC1 depletion on p27Kip1 degradation and cell-cycle progression.

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