Article abstract
Nature Cell Biology 6, 1071 - 1081 (2004)
Published online: 24 October 2004 | doi:10.1038/ncb1180
Secretory traffic triggers the formation of tubular continuities across Golgi sub-compartments
Alvar Trucco1,3, Roman S. Polishchuk1,3, Oliviano Martella1, Alessio Di Pentima1, Aurora Fusella1, Daniele Di Giandomenico1, Enrica San Pietro1, Galina V. Beznoussenko1, Elena V. Polishchuk1, Massimiliano Baldassarre1, Roberto Buccione1, Willie J. C. Geerts2, Abraham J. Koster2, Koert N. J. Burger2, Alexander A. Mironov1 & Alberto Luini1
Abstract
The organization of secretory traffic remains unclear, mainly because of the complex structure and dynamics of the secretory pathway. We have thus studied a simplified system, a single synchronized traffic wave crossing an individual Golgi stack, using electron tomography. Endoplasmic-reticulum-to-Golgi carriers join the stack by fusing with cis cisternae and induce the formation of intercisternal tubules, through which they redistribute their contents throughout the stack. These tubules seem to be pervious to Golgi enzymes, whereas Golgi vesicles are depleted of both enzymes and cargo. Cargo then traverses the stack without leaving the cisternal lumen. When cargo exits the stack, intercisternal connections disappear. These findings provide a new view of secretory traffic that includes dynamic intercompartment continuities as key players.
- Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, 66030 Santa Maria Imbaro (Chieti), Italy.
- Department of Molecular Cell Biology, Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands.
- These authors contributed equally to this work.
Correspondence to: Alexander A. Mironov1 e-mail: mironov@dcbo.negrisud.it
Correspondence to: Alberto Luini1 e-mail: luini@negrisud.it
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