Abstract

It's a common frustration that most readers have probably experienced at one time or another: you see an intriguing image in a paper, but want to know just that bit more than is described. Just how bright is bright: what were the microscope settings when the image was taken? How many sections were used to demonstrate the apparent colocalization of two proteins? Where is the particle moving between two time-points? Our increasing reliance on live imaging analysis for cell biological research and the digital processing that most imaging data undergo before publication brings this issue to the forefront of our minds (also see Rossner, M. & Yamada, K. J. Cell Biol.