Supplementary information

From the following article

Cdk5 is essential for synaptic vesicle endocytosis

Timothy C. Tan, Valentina A. Valova, Chandra S. Malladi, Mark E. Graham, Leise A. Berven, Orla J. Jupp, Gurdip Hansra, Sonya J. McClure, Boris Sarcevic, Ross A. Boadle, Martin R. Larsen, Michael A. Cousin & Phillip J. Robinson

Nature Cell Biology 5, 701 - 710 (2003) Published online: 13 July 2003

doi:10.1038/ncb1020

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Supplementary Information Fig. S1-S6

Supplementary Information Fig. S1a: Effect of cdk5 phosphorylation on dynamin's GTPase activity.

Supplementary Information Fig. S2: Roscovitine inhibits dynamin I phosphorylation by cdk5 but not by PKC-alpha in vitro. is presented. Arrows indicate either dynamin I (Dyn) or synapsin I (Syn, doublet).

Supplementary Information Fig. S3: Lack of effect of PD 98059 on dynamin I phosphorylation and SVE.

Supplementary Information Fig. S4: Double pull-down of 4 dephosphins and synapsin from synaptosomes.

Supplementary Information Fig. S5; Morphology of synaptosomes at rest or after two cycles of stimulus- recovery.

Supplementary Information Fig. S6: Morphology of synaptosomes during S2 in the presence of roscovitine.

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