 | Figure 1
Nature Cell Biology
5, 711 - 719 (2003)
Published online: 6 July 2003; | doi:10.1038/ncb1019
Differing modes of tumour cell invasion have distinct requirements for Rho/ROCK signalling and extracellular proteolysisErik Sahai
& Christopher J. Marshall | | | | Figure 1. Different morphologies of tumour cells in a 3D matrix in vitro and in vivo.
(a) Tumour cells that had invaded a layer of Matrigel were imaged by confocal microscopy. Panels show a 3D reconstruction generated using volocity software from at least 20 confocal sections. Phalloidin staining (F-actin) is in red and cortactin staining in green. Scale bars represent 20  m. (b) GFP-expressing tumour cells grown as a tumour xenograft were imaged ex vivo by capturing at least 20 confocal sections. These were subsequently reconstructed in three dimensions using volocity software (tumour cells shown in green). In addition, A375m2 cells were stained for active caspase 3 (red). Yellow arrowhead indicates an apoptotic cell. White arrows show elongated protrusions. Scale bars represent 20 m in all panels. Inset panel shows only active caspase staining.
|
| | | |  |
|
