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Letter

Nature Cell Biology 5, 572–577 (1 June 2003) | doi:10.1038/ncb997

Colocalization of multiple DNA double-strand breaks at a single Rad52 repair centre

Michael Lisby , Uffe H. Mortensen & Rodney Rothstein

DNA double-strand break repair (DSBR) is an essential process for preserving genomic integrity in all organisms. To investigate this process at the cellular level, we engineered a system of fluorescently marked DNA double-strand breaks (DSBs) in the yeast Saccharomyces cerevisiae to visualize in vivo DSBR in single cells.