Letter abstract
Nature Cell Biology 5, 572 - 577 (2003)
Published online: 19 May 2003 | doi:10.1038/ncb997
Colocalization of multiple DNA double-strand breaks at a single Rad52 repair centre
Michael Lisby1, Uffe H. Mortensen2 & Rodney Rothstein1
DNA double-strand break repair (DSBR) is an essential process for preserving genomic integrity in all organisms. To investigate this process at the cellular level, we engineered a system of fluorescently marked DNA double-strand breaks (DSBs) in the yeast Saccharomyces cerevisiae to visualize in vivo DSBR in single cells. Using this system, we demonstrate for the first time that Rad52 DNA repair foci and DSBs colocalize. Time-lapse microscopy reveals that the relocalization of Rad52 protein into a focal assembly is a rapid and reversible process. In addition, analysis of DNA damage checkpoint-deficient cells provides direct evidence for coordination between DNA repair and subsequent release from checkpoint arrest. Finally, analyses of cells experiencing multiple DSBs demonstrate that Rad52 foci are centres of DNA repair capable of simultaneously recruiting more than one DSB.
- Department of Genetics & Development, Columbia University, College of Physicians & Surgeons, 701 West 168th Street, New York, NY 10032-2704, USA.
- Center for Process Biotechnology, BioCentrum-DTU, Technical University of Denmark, Bldg. 223, DK-2800 Lyngby, Denmark.
Correspondence to: Rodney Rothstein1 e-mail: rothstein@cancercenter.columbia.edu
|
MORE ARTICLES LIKE THIS These links to content published by NPG are automatically generated RESEARCH |
