Article abstract
Nature Cell Biology 5, 195 - 204 (2003)
Published online: 10 February 2003 | doi:10.1038/ncb935
There is a Corrigendum (July 2003) associated with this Article.
RhoD regulates endosome dynamics through Diaphanous-related Formin and Src tyrosine kinase
Stéphane Gasman1,2, Yannis Kalaidzidis3 & Marino Zerial1
Abstract
Early endosomes move bidirectionally between the cell periphery and the interior through a mechanism regulated by the low molecular weight GTPase RhoD. Here, we identify a novel splice variant of human Diaphanous, hDia2C, which specifically binds to RhoD and is recruited onto early endosomes. Expression of RhoD and hDia2C induces a striking alignment of early endosomes along actin filaments and reduces their motility. This activity depends on the membrane recruitment and activation of c-Src kinase, thus uncovering a new role in endosome function. Our results define a novel signal transduction pathway, in which hDia2C and c-Src are sequentially activated by RhoD to regulate the motility of early endosomes through interactions with the actin cytoskeleton.
- Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, Dresden D-01307, Germany
- CNRS UPR-2356 Neurotransmission et Sécrétion Neuroendocrine, 5 rue Blaise Pascal, 67084 Strasbourg, France
- A.N. Belozersky Institute of Physico-chemical Biology, Build A, Moscow State University, Moscow 119899, Russia
Correspondence to: Marino Zerial1 e-mail: zerial@mpi-cbg.de
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