Letter abstract
Nature Cell Biology 5, 1111 - 1116 (2003)
Published online: 16 November 2003 | doi:10.1038/ncb1069
Hsk1–Dfp1 is required for heterochromatin-mediated cohesion at centromeres
Julie M. Bailis1, Pascal Bernard2,3, Richard Antonelli2,4, Robin C. Allshire2,4 & Susan L. Forsburg1
Heterochromatin performs a central role in chromosome segregation and stability by promoting cohesion at centromeres1, 2. Establishment of both heterochromatin-mediated silencing and cohesion requires passage through S phase, although the mechanism is unknown3, 4. Here we demonstrate that Schizosaccharomyces pombe Hsk1 (CDC7), a conserved Dbf4-dependent protein kinase (DDK) that regulates replication initiation5, interacts with and phosphorylates the heterochromatin protein 1 (HP1) equivalent Swi6 (ref. 6). Hsk1 and its regulatory subunit Dfp1 function downstream of Swi6 localization to promote heterochromatin function and cohesion specifically at centromeres. This role for Hsk1–Dfp1 is separable from its replication initiation activity, providing a temporal link between S phase and centromere cohesion that is mediated by heterochromatin.
- Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
- MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK.
- Institut de Biochimie et Génétique Cellulaires, CNRS, Unité Mixte de Recherche 5095, 1 Rue Camille Saint Saëns, 33077, Bordeaux Cedex, France.
- The Wellcome Trust Centre for Cell Biology, ICMB, University of Edinburgh, The King's Buildings, Edinburgh, EH9 3JR, UK.
Correspondence to: Susan L. Forsburg1 e-mail: forsburg@salk.edu
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