Journal home
Advance online publication
Current issue
Archive
Press releases
Supplements and Focuses
Image gallery
Guide to authors
Online submissionOnline submission
Permissions
For referees
Free online issue
Contact the journal
Subscribe
Advertising
work@npg
naturereprints
About this site
For librarians
 
NPG Resources
Nature
Nature Reviews Molecular Cell Biology
UCSD-Nature Signaling Gateway
The Cell Migration Gateway
Nature Reports Stem Cells
Nature Reports Avian Flu
NPG Subject areas
Biotechnology
Cancer
Chemistry
Clinical Medicine
Dentistry
Development
Drug Discovery
Earth Sciences
Evolution & Ecology
Genetics
Immunology
Materials Science
Medical Research
Microbiology
Molecular Cell Biology
Neuroscience
Pharmacology
Physics
Browse all publications
Letter
Nature Cell Biology  5, 928 - 935 (2003)
Published online: 21 September 2003; | doi:10.1038/ncb1046

S-phase checkpoint controls mitosis via an APC-independent Cdc20p function

Duncan J. Clarke1, 2, Marisa Segal1, 3, Catherine A. Andrews2, Stanislav G. Rudyak1, Sanne Jensen1, 4, Karen Smith2 & Steven I. Reed1

1  The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.

2  Department of Genetics, Cell Biology and Development, University of Minnesota Medical School, 420 Washington Avenue SE, Minneapolis, Minnesota 55455, USA.

3  Present address: Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.

4  Present address: Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, 2100 Copenhagen, Denmark.

Correspondence should be addressed to Steven I. Reed sreed@scripps.edu
Cells divide with remarkable fidelity, allowing complex organisms to develop and possess longevity. Checkpoint controls contribute by ensuring that genome duplication and segregation occur without error so that genomic instability, associated with developmental abnormalities and a hallmark of most human cancers1, 2, 3, 4, 5, is avoided. S-phase checkpoints prevent cell division while DNA is replicating6, 7, 8. Budding yeast Mec1p and Rad53p, homologues of human checkpoint kinases ATM/ATR and Chk2, are needed for this control system. How Mec1p and Rad53p prevent mitosis in S phase is not known. Here we provide evidence that budding yeasts avoid mitosis during S phase by regulating the anaphase-promoting complex (APC) specificity factor Cdc20p: Mec1p and Rad53p repress the accumulation of Cdc20p in S phase. Because precocious Cdc20p accumulation causes anaphase onset and aneuploidy, Cdc20p concentrations must be precisely regulated during each and every cell cycle. Catastrophic mitosis induced by Cdc20p in S phase occurs even in the absence of core APC components. Thus, Cdc20p can function independently of the APC.


MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated

REFERENCE
Checkpoints in the Cell Cycle
Nature Encyclopaedia of Life Sciences

REVIEWS
Cell death by mitotic catastrophe: a molecular definition
Oncogene Reviews (12 Apr 2004)
 See all 3 matches for Reviews

NEWS AND VIEWS
Can Fizzy fly solo?
Nature Cell Biology News and Views (01 Oct 2003)
Cell cycle: Dual control of mitotic exit
Nature News and Views (11 Nov 1999)
 See all 4 matches for News And Views

RESEARCH
Mec1p regulates Pds1p levels in S phase: complex coordination of DNA replication and mitosis
Nature Cell Biology Article (01 Jul 2001)
 See all 33 matches for Research

 Top
Abstract
Previous
Table of contents
Full textFull text
Download PDFDownload PDF
Send to a friendSend to a friend
Save this linkSave this link

Open Innovation Challenges

  • Corrosion Inhibitor

    • Deadline: Aug 19 2009
    • Reward: $10,000 USD

    The Seeker is looking for inhibitors of corrosion. This Challenge requires only a written descripti...

  • Efficient Chromosome Doubling: Plant Cell Division

    • Deadline: Jul 15 2009
    • Reward: $20,000 USD

    The Seeker is looking for an efficient chromosome doubling method in plants and in particular, metho...

naturejobs

Figures & Tables
Supplementary info
See also: News and Views by Jackson
Export citation
natureproducts

Search buyers guide:

 
ADVERTISEMENT
 
Nature Cell Biology
ISSN: 1465-7392
EISSN: 1476-4679
Journal home | Advance online publication | Current issue | Archive | Press releases | For authors | Online submission | For referees | Free online issue | About the journal | Contact the journal | Subscribe | Advertising | work@npg | Reprints and permissions | About this site | For librarians
Nature Publishing Group, publisher of Nature, and other science journals and reference works©2003 Nature Publishing Group | Privacy policy