Figure 2 - rpr induces post-transcriptional downregulation of DIAP1.

With the exception of a, anterior is to the left and posterior to the right. The A–P boundary is outlined in a, b and g. a–d, rpr and p35 were ectopically expressed in the wing imaginal disc posterior compartment. Genotype, UAS-rpr; UAS-p35/en-Gal4. a, p35 completely blocks rpr-induced cell death and flies develop wings with minimal defects. Anterior (A) is up and posterior (P) down. b, In the anterior compartment, the CM1 antibody (red) intensely labels naturally dying cells (arrow). In the posterior compartment, all cells are labelled with CM1, but staining was confined to the cytoplasm. c,d, Wing imaginal discs labelled with the anti-DIAP1 antibody (green) show reduced DIAP1 levels in the posterior compartment. The posterior compartment is marked by double labelling with the anti-EN antibody (c, blue). e, Expression of p35 alone does not affect DIAP1 levels (green). Genotype, UAS-p35/en-Gal4. f, hid and p35 were co-expressed in the posterior compartment and labelled with the anti-DIAP1 antibody (green). Genotype, UAS-hid; UAS-p35/en-Gal4. Compared to c and d, hid does not induce an obvious reduction of DIAP1 levels. g, diap1-lacZ (th^j5c8) expression (red) is higher in the posterior compartment, where rpr is expressed. This indicates that the observed reduction of DIAP1 in c and d is post-transcriptional. Genotype, UAS-rpr/+; UAS-p35/en-Gal4; diap1-lacZ (th^j5c8)/+.