Table of contents
Editorial
An integrative future - pE109
doi:10.1038/ncb0502-e109
Full Text - An integrative future | PDF (54 KB) - An integrative future
Letters to Editor
Chronic granulomatous disease mutations and the PX domain - pE110
Paul G. Heyworth & Andrew R. Cross
doi:10.1038/ncb0502-e110a
Full Text - Chronic granulomatous disease mutations and the PX domain | PDF (465 KB) - Chronic granulomatous disease mutations and the PX domain
Amyloid-lowering isocoumarins are not direct inhibitors of 
-secretase - ppE110 - E111
William P. Esler, Chittaranjan Das, William A. Campbell, W. Taylor Kimberly, Anna Y. Kornilova, Thekla S. Diehl, Wenjuan Ye, Beth L. Ostaszewski, Weiming Xia, Dennis J. Selkoe & Michael S. Wolfe
doi:10.1038/ncb0502-e110b
Full Text - Amyloid-lowering isocoumarins are not direct inhibitors of 
-secretase | PDF (465 KB) - Amyloid-lowering isocoumarins are not direct inhibitors of -secretase
Amyloid-lowering isocoumarins are not direct inhibitors of -secretase - Reponse - ppE111 - E112
Agnes Petit, Cécile Dumanchin-Njock, David Andrau, Cristine Alves da Costa & Frédéric Checler
doi:10.1038/ncb0502-e110c
Full Text - Amyloid-lowering isocoumarins are not direct inhibitors of -secretase - Reponse | PDF (465 KB) - Amyloid-lowering isocoumarins are not direct inhibitors of -secretase - Reponse
Commentary
Taking a bite: proteasomal protein processing - ppE113 - E116
Michael Rape & Stefan Jentsch
doi:10.1038/ncb0502-e113
The proteasome is a hollow cylindrical protease that contains active sites concealed within its central cavity. Proteasomes usually completely degrade substrates into small peptides, but in a few cases, degradation can yield biologically active protein fragments. Examples of this are the transcription factors NF-
B, Spt23p and Mga2p, which are generated from precursors by proteasomal processing. How distinct protein domains are spared from degradation remains a matter of debate. Here, we discuss several models and suggest a novel mechanism for proteasomal processing.
Full Text - Taking a bite: proteasomal protein processing | PDF (281 KB) - Taking a bite: proteasomal protein processing
News and Views
Cell polarity: Oskar seeks PARtner for a stable relationship - ppE117 - E118
Simon L. Bullock & David Ish-Horowicz
doi:10.1038/ncb0502-e117
The Par-1/MARK kinases have a conserved role in cell polarization and are required to establish the anterior–posterior axis of Caenorhabditis elegans and Drosophila melanogaster. Now, elegant studies in Drosophila uncover the posterior patterning molecule Oskar (Osk) as a direct target of Par-1. Phosphorylation of Oskar at the posterior pole stabilizes the protein and contributes to its localized accumulation at high levels.
Full Text - Cell polarity: Oskar seeks PARtner for a stable relationship | PDF (106 KB) - Cell polarity: Oskar seeks PARtner for a stable relationship
See also: Article by Riechmann et al.
Conspiracy to disarm APC in interphase - ppE119 - E120
Hartmut C. Vodermaier & Jan-Michael Peters
doi:10.1038/ncb0502-e119
The anaphase-promoting complex (APC) initiates exit from mitosis by ubiquitinating A- and B-type cyclins, the activating subunits of cyclin-dependent kinase 1 (Cdk1). Subsequently, the APC has to be inactivated to allow the re-accumulation of mitotic cyclins in the next cell cycle. A newly identified inhibitor of the APC, called Emi1 in vertebrates and Rca1 in Drosophila melanogaster, may have an important function in inactivating the APC during interphase.
Full Text - Conspiracy to disarm APC in interphase | PDF (90 KB) - Conspiracy to disarm APC in interphase
See also: Article by Hsu et al.
Ubiquitin chained and crosslinked - ppE121 - E123
Daniel Finley
doi:10.1038/ncb0502-e121
Polyubiquitin chains are assembled onto proteins destined for degradation. The target protein is then unfolded by the proteasome and translocated through a channel leading from the unfolding site to an internal chamber of the enzyme for hydrolysis. A recent paper illuminates the long-elusive polyubiquitin chain recognition step that initiates this sequence of events at the proteasome.
Full Text - Ubiquitin chained and crosslinked | PDF (414 KB) - Ubiquitin chained and crosslinked
An encore for kiss and run? - pE123
Barbara Marte
doi:10.1038/ncb0502-e123
Full Text - An encore for kiss and run? | PDF (169 KB) - An encore for kiss and run?
Getting legless with a Pygopus - pE124
Sarah Greaves
doi:10.1038/ncb0502-e124
Full Text - Getting legless with a Pygopus | PDF (133 KB) - Getting legless with a Pygopus
See also: Article by Thompson et al.
Book Reviews
The forgotten chaperones - pE125
Eric Solary & Carmen Garrido review Small Stress Proteins by A. P. Arrigo & W. E. G. Müller
doi:10.1038/ncb0502-e125
Full Text - The forgotten chaperones | PDF (92 KB) - The forgotten chaperones
The role of cytometry in research and patient care - pE126
Rafael Nunez reviews Cytometric analysis of cell phenotype and function by D. A. McCarthy & M. G. Macey
doi:10.1038/ncb0502-e126
Full Text - The role of cytometry in research and patient care | PDF (156 KB) - The role of cytometry in research and patient care
Historical Perspective
The origins of protein phosphorylation - ppE127 - E130
Philip Cohen
doi:10.1038/ncb0502-e127
Abstract - | Full Text - The origins of protein phosphorylation | PDF (180 KB) - The origins of protein phosphorylation
Review
AP-1 as a regulator of cell life and death - ppE131 - E136
Eitan Shaulian & Michael Karin
doi:10.1038/ncb0502-e131
Abstract - | Full Text - AP-1 as a regulator of cell life and death | PDF (143 KB) - AP-1 as a regulator of cell life and death
Articles
The TRPM7 channel is inactivated by PIP2 hydrolysis - pp329 - 336
Loren W. Runnels, Lixia Yue & David E. Clapham
doi:10.1038/ncb781
Abstract - | Full Text - The TRPM7 channel is inactivated by PIP2 hydrolysis | PDF (1,104 KB) - The TRPM7 channel is inactivated by PIP2 hydrolysis
Par-1 regulates stability of the posterior determinant Oskar by phosphorylation - pp337 - 342
Veit Riechmann, Gustavo J. Gutierrez, Paolo Filardo, Angel R. Nebreda & Anne Ephrussi
doi:10.1038/ncb782
Abstract - | Full Text - Par-1 regulates stability of the posterior determinant Oskar by phosphorylation | PDF (832 KB) - Par-1 regulates stability of the posterior determinant Oskar by phosphorylation | Supplementary information
See also: News and Views by Bullock & Ish-Horowicz
Ras signalling on the endoplasmic reticulum and the Golgi - pp343 - 350
Vi K. Chiu, Trever Bivona, Angela Hach, J. Bernard Sajous, Joseph Silletti, Heidi Wiener, Ronald L. Johnson, II, Adrienne D. Cox & Mark R. Philips
doi:10.1038/ncb783
Abstract - | Full Text - Ras signalling on the endoplasmic reticulum and the Golgi | PDF (2,719 KB) - Ras signalling on the endoplasmic reticulum and the Golgi | Supplementary information
Frodo interacts with Dishevelled to transduce Wnt signals - pp351 - 357
Joachim Gloy, Hiroki Hikasa & Sergei Y. Sokol
doi:10.1038/ncb784
Abstract - | Full Text - Frodo interacts with Dishevelled to transduce Wnt signals | PDF (712 KB) - Frodo interacts with Dishevelled to transduce Wnt signals
E2F-dependent accumulation of hEmi1 regulates S phase entry by inhibiting APCCdh1 - pp358 - 366
Jerry Y. Hsu, Julie D.R. Reimann, Claus S. Sørensen, Jiri Lukas & Peter K. Jackson
doi:10.1038/ncb785
Abstract - | Full Text - E2F-dependent accumulation of hEmi1 regulates S phase entry by inhibiting APCCdh1 | PDF (1,209 KB) - E2F-dependent accumulation of hEmi1 regulates S phase entry by inhibiting APCCdh1 | Supplementary information
See also: News and Views by Vodermaier & Peters
A new nuclear component of the Wnt signalling pathway - pp367 - 373
Barry Thompson, Fiona Townsley, Rina Rosin-Arbesfeld, Hannah Musisi & Mariann Bienz
doi:10.1038/ncb786
Abstract - | Full Text - A new nuclear component of the Wnt signalling pathway | PDF (2,514 KB) - A new nuclear component of the Wnt signalling pathway | Supplementary information
See also: News and Views by Greaves
Brief Communications
A distinct class of endosome mediates clathrin-independent endocytosis to the Golgi complex - pp374 - 378
Benjamin J. Nichols
doi:10.1038/ncb787
Abstract - | Full Text - A distinct class of endosome mediates clathrin-independent endocytosis to the Golgi complex | PDF (3,282 KB) - A distinct class of endosome mediates clathrin-independent endocytosis to the Golgi complex | Supplementary information
Dysfunction of store-operated calcium channel in muscle cells lacking mg29 - pp379 - 383
Zui Pan, Dongmei Yang, Ramakrishnan Y. Nagaraj, Thomas A. Nosek, Miyuki Nishi, Hiroshi Takeshima, Heping Cheng & Jianjie Ma
doi:10.1038/ncb788
Abstract - | Full Text - Dysfunction of store-operated calcium channel in muscle cells lacking mg29 | PDF (553 KB) - Dysfunction of store-operated calcium channel in muscle cells lacking mg29
Maintenance of replication forks and the S-phase checkpoint by Cdc18p and Orp1p - pp384 - 388
Hiroshi Murakami, Stephanie K. Yanow, Dominic Griffiths, Makoto Nakanishi & Paul Nurse
doi:10.1038/ncb789
Abstract - | Full Text - Maintenance of replication forks and the S-phase checkpoint by Cdc18p and Orp1p | PDF (152 KB) - Maintenance of replication forks and the S-phase checkpoint by Cdc18p and Orp1p | Supplementary information
Epsins and Vps27p/Hrs contain ubiquitin-binding domains that function in receptor endocytosis - pp389 - 393
Susan C. Shih, David J. Katzmann, Joshua D. Schnell, Myra Sutanto, Scott D. Emr & Linda Hicke
doi:10.1038/ncb790
Abstract - | Full Text - Epsins and Vps27p/Hrs contain ubiquitin-binding domains that function in receptor endocytosis | PDF (295 KB) - Epsins and Vps27p/Hrs contain ubiquitin-binding domains that function in receptor endocytosis | Supplementary information
Hrs sorts ubiquitinated proteins into clathrin-coated microdomains of early endosomes - pp394 - 398
Camilla Raiborg, Kristi G. Bache, David J. Gillooly, Inger Helene Madshus, Espen Stang & Harald Stenmark
doi:10.1038/ncb791
Abstract - | Full Text - Hrs sorts ubiquitinated proteins into clathrin-coated microdomains of early endosomes | PDF (1,830 KB) - Hrs sorts ubiquitinated proteins into clathrin-coated microdomains of early endosomes | Supplementary information
