Article abstract
Nature Cell Biology 4, 279 - 285 (2002)
Published online: 18 March 2002 | doi:10.1038/ncb771
Quantal release of free radicals during exocytosis of phagosomes
Anke Di1, Boris Krupa1, Vytas P. Bindokas1, Yimei Chen2, Mary E. Brown1, H. Clive Palfrey1, Anjaparavanda P. Naren3, Kevin L. Kirk3 & Deborah J. Nelson1
Abstract
Secretion of lysosomes and related organelles is important for immune system function. High-resolution membrane capacitance techniques were used to track changes in membrane area in single phagocytes during opsonized polystyrene bead uptake and release. Secretagogue stimulation of cells preloaded with beads resulted in immediate vesicle discharge, visualized as step increases in capacitance. The size of the increases were consistent with phagosome size. This hypothesis was confirmed by direct observation of dye release from bead-containing phagosomes after secretagogue stimulation. Capacitance recordings of exocytosis were correlated with quantal free radical release, as determined by amperometry. Thus, phagosomes undergo regulated secretion in macrophages, one function of which may be to deliver sequestered free radicals to the extracellular space.
- Dept. of Neurobiology, Pharmacology and Physiology, The University of Chicago, Chicago, IL 60637, USA
- Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA
- Department of Physiology and Biophysics, Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, 1918 University Blvd., MCLM 985, Birmingham, AL 35294, USA
Correspondence to: Deborah J. Nelson1 e-mail: dnelson@drugs.bsd.uchicago.edu
|
MORE ARTICLES LIKE THIS These links to content published by NPG are automatically generated REFERENCE REVIEWS NEWS AND VIEWS RESEARCH |
