Figure 1 - The WAVE-1 protein network and characterization of WRP.

WAVE-1 immune complexes were isolated from rat brain extracts. a, Pre-immune (lane 1) and WAVE immunoprecipitations (lane 2) were resolved by SDS–PAGE and detected by silver staining. Bands specific to the WAVE immune complexes were numbered, excised from the gel and digested with trypsin; the resulting peptides sequenced by nanoelectrospray tandem mass spectrometry. b, Representative example of data obtained from band 4 contained in EST clone KIAA0411 (WRP).c, Amino-acid sequence of WRP. Each peptide identified by mass spectrometry is highlighted. d, Domain structure of WRP, showing the location of an FCH domain, a region with homology to Rho-family GAP proteins and an SH3 domain. e, Tissue expression of WRP determined by northern blot using KIAA0411 as a probe. PBL, peripheral blood leukocyte. f, Expression pattern of WRP mRNA in selected brain regions (top) was compared with that of WAVE-1 (bottom). g, h, Confirmation of the WRP–WAVE interaction by reciprocal co-immunoprecipitation techniques. g, Immunodetection of WRP in WAVE complexes from rat brain. h, Immunodetection of WAVE in WRP complexes from rat brain.