Article abstract
Nature Cell Biology 4, 955 - 963 (2002)
Published online: 25 November 2002 | doi:10.1038/ncb888
Regulated exocytosis: a novel, widely expressed system
Barbara Borgonovo1, Emanuele Cocucci1,2, Gabriella Racchetti1,3, Paola Podini1,3, Angela Bachi3,4 & Jacopo Meldolesi1,3
Abstract
Electrophysiological studies in some secretory and non-secretory cells have identified an extensive form of calcium-induced exocytosis that is rapid (hundreds of milliseconds), insensitive to tetanus toxin and distinct from regulated secretion. We have now identified a marker of the process, desmoyokin-AHNAK, in a clonal derivative of the neuronal cell line, PC12. In resting cells, desmoyokin-AHNAK is localized within the lumen of specific vesicles, but appears on the cell surface during stimulation. Desmoyokin-AHNAK-positive vesicles exist in a variety of cells and tissues and are distinct from the endoplasmic reticulum, Golgi, trans-Golgi, endosomes and lysosomes, and from Glut4 and constitutive secretion vesicles. They seem to be involved in two models of plasmalemma enlargement: differentiation and membrane repair. We therefore propose that these vesicles should be called 'enlargosomes'.
- Department of Neuroscience, Vita-Salute San Raffaele University and Excellence Centre in Cell Differentiation, via Olgettina 58, 20132 Milan, Italy
- Department of Pharmacology, University of Milan, via Vanvitelli 32, 20133 Milan, Italy
- IRCCS San Raffaele, Dibit, via Olgettina 58, 20132 Milan, Italy
- Department of Cell Pathology, Vita-Salute San Raffaele University and Excellence Centre in Cell Differentiation, via Olgettina 58, 20132 Milan, Italy
Correspondence to: Jacopo Meldolesi1,3 e-mail: meldolesi.jacopo@hsr.it
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