Nature Cell Biology4, 970 - 975 (2002)
Published online: 25 November 2002; | doi:10.1038/ncb886
The WRP component of the WAVE-1 complex attenuates Rac-mediated signalling
Scott H. Soderling1, 5, Kathleen L. Binns5, 2, 3, Gary A. Wayman4, Stephen M. Davee1, Siew Hwa Ong2, Tony Pawson3
& John D. Scott1
1
Howard Hughes Medical Institute, Vollum Institute, 3181 Sam Jackson Park Road, Portland, Oregon 97239-3098, USA
2
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
3
Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario M5G 1X5, Canada
4
Vollum Institute, 3181 Sam Jackson Park Road, Portland, Oregon 97239-3098, USA
5
These authors contributed equally to this work
Correspondence should be addressed to John D. Scott scott@ohsu.edu
WAVE-1, which is also known as Scar, is a scaffolding protein that directs actin reorganization by relaying signals from the GTPase Rac to the Arp2/3 complex. Although the molecular details of WAVE activation by Rac have been described, the mechanisms by which these signals are terminated remain unknown. Here we have used tandem mass spectrometry to identify previously unknown components of the WAVE signalling network including WRP, a Rac-selective GTPase-activating protein. WRP binds directly to WAVE-1 through its Src homology domain 3 and specifically inhibits Rac function in vivo. Thus, we propose that WRP is a binding partner of WAVE-1 that functions as a signal termination factor for Rac.
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