De novo formation of transitional ER sites and Golgi structures in Pichia pastoris

Movie 1 (mov 4,913)
P. pastoris cells containing Sec13p-GFP-labeled tER sites were imaged for ~ 33 min. Several de novo formation and fusion events can be seen. Weak nuclear fluorescence is also visible. This movie is accelerated 60-fold relative to real time.

Movie 2 (mov 3,598)
P. pastoris cells containing Sec13p-GFP-labeled tER sites were imaged for ~ 30 min. Several de novo formation and fusion events can be seen, particularly in the cell on the lower left. Weak nuclear fluorescence is also visible. This movie is accelerated 60-fold relative to real time.

Movie 3 (mov 1,333)
P. pastoris cells containing Sec13p-GFP-labeled tER sites and Sec7p-DsRed-labeled Golgi structures were imaged for ~ 14 min. The formation and growth of new Golgi structures is linked to that of the associated tER sites, as seen most clearly in the pair of cells on the left. This movie is accelerated 60-fold relative to real time.

Movie 4 (mov 904)
P. pastoris cells containing Sec13p-GFP-labeled tER sites and Sec7p-DsRed-labeled Golgi structures were imaged for ~ 15 min. In the cell on the left, two tER sites fuse, and the associated Golgi structures also fuse. This movie is accelerated 60-fold relative to real time.

Movie 5 (mov 2,645)
P. pastoris cells containing GFP-Gos1p-labeled Golgi structures were imaged for ~ 21 min. Several de novo formation and fusion events can be seen, particularly in the cell on the lower left. This movie is accelerated 60-fold relative to real time.

Movie 6 (mov 240)
P. pastoris cells containing containing Sec13p-GFP-labeled tER sites and Sec7p-DsRed-labeled Golgi structures were imaged for ~ 4 min. In the cell on the left, a Golgi structure moves away from its associated tER site toward the bud. This movie is accelerated 12-fold relative to real time.

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