Article abstract
Nature Cell Biology 4, 766 - 773 (2002)
Published online: 30 September 2002 | doi:10.1038/ncb854
Elimination of host cell PtdIns(4,5)P2 by bacterial SigD promotes membrane fission during invasion by Salmonella
Mauricio R. Terebiznik1, Otilia V. Vieira1, Sandra L. Marcus2, Andrea Slade3, Christopher M. Yip3,4, William S. Trimble1, Tobias Meyer5, B. Brett Finlay2 & Sergio Grinstein1
Abstract
Salmonella invades mammalian cells by inducing membrane ruffling and macropinocytosis through actin remodelling. Because phosphoinositides are central to actin assembly, we have studied the dynamics of phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2) in HeLa cells during invasion by Salmonella typhimurium. Here we show that the outermost parts of the ruffles induced by invasion show a modest enrichment in PtdIns(4,5)P2, but that PtdIns(4,5)P2 is virtually absent from the invaginating regions. Rapid disappearance of PtdIns(4,5)P2 requires the expression of the Salmonella phosphatase SigD (also known as SopB). Deletion of SigD markedly delays fission of the invaginating membranes, indicating that elimination of PtdIns(4,5)P2 may be required for rapid formation of Salmonella-containing vacuoles. Heterologous expression of SigD is sufficient to promote the disappearance of PtdIns(4,5)P2, to reduce the rigidity of the membrane skeleton, and to induce plasmalemmal invagination and fission. Hydrolysis of PtdIns(4,5)P2 may be a common and essential feature of membrane fission during several internalization processes including invasion, phagocytosis and possibly endocytosis.
- Cell Biology Program, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
- Biotechnology Laboratory, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada
- Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 3G9, Canada
- Department of Chemical Engineering, University of Toronto, Toronto, Ontario M5S 3G9, Canada
- Department of Molecular Pharmacology, Stanford University, Stanford, California, USA
Correspondence to: Sergio Grinstein1 e-mail: sga@sickkids.ca
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