Article abstract
Nature Cell Biology 4, 750 - 756 (2002)
Published online: 23 September 2002 | doi:10.1038/ncb852
De novo formation of transitional ER sites and Golgi structures in Pichia pastoris
Brooke J. Bevis1, Adam T. Hammond1, Catherine A. Reinke1 & Benjamin S. Glick1
Abstract
Transitional ER (tER) sites are ER subdomains that are functionally, biochemically and morphologically distinct from the surrounding rough ER. Here we have used confocal video microscopy to study the dynamics of tER sites and Golgi structures in the budding yeast Pichia pastoris. The biogenesis of tER sites is tightly linked to the biogenesis of Golgi, and both compartments can apparently form de novo. tER sites often fuse with one another, but they maintain a consistent average size through shrinkage after fusion and growth after de novo formation. Golgi dynamics are similar, although late Golgi elements often move away from tER sites towards regions of polarized growth. Our results can be explained by assuming that tER sites give rise to Golgi cisternae that continually mature.
- Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA
Correspondence to: Benjamin S. Glick1 e-mail: bsglick@midway.uchicago.edu
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