Advance online publication - pE1

doi:10.1038/ncb0102-e1

Full Text - Advance online publication | PDF (59 KB) - Advance online publication

Lymphatic endothelium: a new frontier of metastasis research - ppE2 - E5

Marika J. Karkkainen, Taija Mäkinen & Kari Alitalo

doi:10.1038/ncb0102-e2

The vascular endothelium is a dynamic tissue with many active functions. Until recently, endothelial cell (EC) biology studies have used cultured ECs from various organs; these cell lines are considered representative of the blood vascular endothelium. Very few lymphatic EC lines have been available, and these were derived from lymphatic tumours or large collecting lymphatic ducts. In the past, lymphatic vessels were defined largely by the lack of erythrocytes in their lumen, a lack of junctional complexes and the lack of a well-defined basement membrane. Now that lymphatic-specific vascular endothelial growth factors (VEGF-C and VEGF-D) and molecular cell surface markers such as the VEGFR-3 receptor have been identified, this definition needs to be updated. Recent developments have highlighted the importance of lymphatic ECs, and they could become the next focus for angiogenesis and metastasis research.

Full Text - Lymphatic endothelium: a new frontier of metastasis research | PDF (669 KB) - Lymphatic endothelium: a new frontier of metastasis research

Strabismus comes into focus - ppE6 - E8

Jeffrey D. Axelrod

doi:10.1038/ncb0102-e6

At least one member of the Frizzled (Fz) family of receptors, together with the downstream signalling protein Dishevelled, participates in several distinct, yet closely related signal transduction pathways. Activation of the correct signal transduction pathway is critical. Here, a new study suggests that the transmembrane protein Strabismus participates in this decision.

Full Text - Strabismus comes into focus | PDF (337 KB) - Strabismus comes into focus

See also: Article by Park & Moon

A crossbridge too far - ppE8 - E10

James A. Spudich & Ronald S. Rock

doi:10.1038/ncb0102-e8

Myosin V is a marvellous molecular motor that delivers various cargo to specific addresses in eukaryotic cells. Recent developments are leading to a detailed molecular understanding of how this enzyme transduces the chemical energy of ATP hydrolysis into mechanical movement along actin filaments.

Full Text - A crossbridge too far | PDF (565 KB) - A crossbridge too far

See also: Article by Veigel et al.

Finding the path less followed - pE10

Alison Schuldt

doi:10.1038/ncb0102-e10

Full Text - Finding the path less followed | PDF (466 KB) - Finding the path less followed

Novel effector function for an old receptor - ppE11 - E12

Rüdiger Klein

doi:10.1038/ncb0102-e11

Integrins are cell surface receptors for extracellular matrix proteins and, as such, regulate adhesive cell–matrix interactions. New findings indicate that the ₄ integrin subunit can also function as a signalling effector for the activated receptor tyrosine kinase Met and enhance invasive growth independent of its adhesive role.

Full Text - Novel effector function for an old receptor | PDF (265 KB) - Novel effector function for an old receptor

Reach for the stars - pE12

Sarah Greaves

doi:10.1038/ncb0102-e12

Full Text - Reach for the stars | PDF (197 KB) - Reach for the stars

Dictyostelium in focus - pE13

Peter A. Thomason reviews Dictyostelium: Evolution, Cell Biology, and the Development of Multicellularity by Richard H. Kessin

doi:10.1038/ncb0102-e13

Full Text - Dictyostelium in focus | PDF (165 KB) - Dictyostelium in focus

Signal transduction according to the book - pE14

John D. Scott reviews Protein Modules in Cellular Signaling by Ludwig Heilmeyer & Peter Friedrich

doi:10.1038/ncb0102-e14

Full Text - Signal transduction according to the book | PDF (119 KB) - Signal transduction according to the book

Imaging into the future: visualizing gene expression and protein interactions with fluorescent proteins - ppE15 - E20

Peter van Roessel & Andrea H. Brand

doi:10.1038/ncb0102-e15

Abstract - | Full Text - Imaging into the future: visualizing gene expression and protein interactions with fluorescent proteins | PDF (273 KB) - Imaging into the future: visualizing gene expression and protein interactions with fluorescent proteins

Regulation of p53 activity by its interaction with homeodomain-interacting protein kinase-2 - pp1 - 10

Thomas G. Hofmann, Andreas Möller, Hüseyin Sirma, Hanswalter Zentgraf, Yoichi Taya, Wulf Dröge, Hans Will & M. Lienhard Schmitz

doi:10.1038/ncb715

Abstract - | Full Text - Regulation of p53 activity by its interaction with homeodomain-interacting protein kinase-2 | PDF (702 KB) - Regulation of p53 activity by its interaction with homeodomain-interacting protein kinase-2 | Supplementary information

Homeodomain-interacting protein kinase-2 phosphorylates p53 at Ser 46 and mediates apoptosis - pp11 - 19

Gabriella D'Orazi, Barbara Cecchinelli, Tiziana Bruno, Isabella Manni, Yuichiro Higashimoto, Shin'ichi Saito, Monica Gostissa, Sabrina Coen, Alessandra Marchetti, Giannino Del Sal, Giulia Piaggio, Maurizio Fanciulli, Ettore Appella & Silvia Soddu

doi:10.1038/ncb714

Abstract - | Full Text - Homeodomain-interacting protein kinase-2 phosphorylates p53 at Ser 46 and mediates apoptosis | PDF (442 KB) - Homeodomain-interacting protein kinase-2 phosphorylates p53 at Ser 46 and mediates apoptosis

The planar cell-polarity gene stbm regulates cell behaviour and cell fate in vertebrate embryos - pp20 - 25

Maiyon Park & Randall T. Moon

doi:10.1038/ncb716

Abstract - | Full Text - The planar cell-polarity gene stbm regulates cell behaviour and cell fate in vertebrate embryos | PDF (1,154 KB) - The planar cell-polarity gene stbm regulates cell behaviour and cell fate in vertebrate embryos

See also: News and Views by Axelrod

Interleukin-12 suppresses ultraviolet radiation-induced apoptosis by inducing DNA repair - pp26 - 31

Agatha Schwarz, Sonja Ständer, Mark Berneburg, Markus Böhm, Dagmar Kulms, Harry van Steeg, Karin Grosse-Heitmeyer, Jean Krutmann & Thomas Schwarz

doi:10.1038/ncb717

Abstract - | Full Text - Interleukin-12 suppresses ultraviolet radiation-induced apoptosis by inducing DNA repair | PDF (264 KB) - Interleukin-12 suppresses ultraviolet radiation-induced apoptosis by inducing DNA repair

Formins direct Arp2/3-independent actin filament assembly to polarize cell growth in yeast - pp32 - 41

Marie Evangelista, David Pruyne, David C. Amberg, Charles Boone & Anthony Bretscher

doi:10.1038/ncb718

Abstract - | Full Text - Formins direct Arp2/3-independent actin filament assembly to polarize cell growth in yeast | PDF (4,532 KB) - Formins direct Arp2/3-independent actin filament assembly to polarize cell growth in yeast

Yeast formins regulate cell polarity by controlling the assembly of actin cables - pp42 - 50

Isabelle Sagot, Saskia K. Klee & David Pellman

doi:10.1038/ncb719

Abstract - | Full Text - Yeast formins regulate cell polarity by controlling the assembly of actin cables | PDF (473 KB) - Yeast formins regulate cell polarity by controlling the assembly of actin cables

TGF- induces apoptosis through Smad-mediated expression of DAP-kinase - pp51 - 58

Chuan-Wei Jang, Chun-Hau Chen, Chun-Chieh Chen, Jia-yun Chen, Yi-Hsien Su & Ruey-Hwa Chen

doi:10.1038/ncb731

Abstract - | Full Text - TGF- induces apoptosis through Smad-mediated expression of DAP-kinase | PDF (402 KB) - TGF- induces apoptosis through Smad-mediated expression of DAP-kinase | Supplementary information

The gated gait of the processive molecular motor, myosin V - pp59 - 65

Claudia Veigel, Fei Wang, Marc L. Bartoo, James R. Sellers & Justin E. Molloy

doi:10.1038/ncb732

Abstract - | Full Text - The gated gait of the processive molecular motor, myosin V | PDF (666 KB) - The gated gait of the processive molecular motor, myosin V

See also: News and Views by Spudich & Rock

The exocyst is a Ral effector complex - pp66 - 72

Serge Moskalenko, Dale O. Henry, Carine Rosse, Gladys Mirey, Jacques H. Camonis & Michael A. White

doi:10.1038/ncb728

Abstract - | Full Text - The exocyst is a Ral effector complex | PDF (554 KB) - The exocyst is a Ral effector complex

The exocyst complex binds the small GTPase RalA to mediate filopodia formation - pp73 - 78

Kazuhiro Sugihara, Shiro Asano, Kenichi Tanaka, Akihiro Iwamatsu, Katsuya Okawa & Yasutaka Ohta

doi:10.1038/ncb720

Abstract - | Full Text - The exocyst complex binds the small GTPase RalA to mediate filopodia formation | PDF (587 KB) - The exocyst complex binds the small GTPase RalA to mediate filopodia formation | Supplementary information

Association of human TFIID–promoter complexes with silenced mitotic chromatin in vivo - pp79 - 82

Rossitza Christova & Thomas Oelgeschläger

doi:10.1038/ncb733

Abstract - | Full Text - Association of human TFIID–promoter complexes with silenced mitotic chromatin in vivo | PDF (333 KB) - Association of human TFIID–promoter complexes with silenced mitotic chromatin in vivo

A small-molecule inhibitor of skeletal muscle myosin II - pp83 - 88

A. Cheung, J. A. Dantzig, S. Hollingworth, S. M. Baylor, Y.E. Goldman, T. J. Mitchison & A. F. Straight

doi:10.1038/ncb734

Abstract - | Full Text - A small-molecule inhibitor of skeletal muscle myosin II | PDF (505 KB) - A small-molecule inhibitor of skeletal muscle myosin II | Supplementary information

Recruitment of cohesin to heterochromatic regions by Swi6/HP1 in fission yeast - pp89 - 93

Nobuhiro Nonaka, Tomoya Kitajima, Shihori Yokobayashi, Guoping Xiao, Masayuki Yamamoto, Shiv I. S. Grewal & Yoshinori Watanabe

doi:10.1038/ncb739

Abstract - | Full Text - Recruitment of cohesin to heterochromatic regions by Swi6/HP1 in fission yeast | PDF (505 KB) - Recruitment of cohesin to heterochromatic regions by Swi6/HP1 in fission yeast | Supplementary information