Article abstract
Nature Cell Biology 3, 816 - 822 (2001)
Published online: 15 August 2001 | doi:10.1038/ncb0901-816
UDP–sugar transporter implicated in glycosylation and processing of Notch
Satoshi Goto1,2,6, Misako Taniguchi1, Masatoshi Muraoka3, Hidenao Toyoda4, Yukiko Sado1, Masao Kawakita6,3 & Shigeo Hayashi1,2,5
Abstract
Glycosylation modifies protein activities in various biological processes. Here, we report the functions of a novel UDP–sugar transporter (UST74C, an alternative name for Fringe connection (Frc)) localized to the Golgi apparatus in cellular signalling of Drosophila. Mutants in the frc gene exhibit phenotypes resembling wingless and Notch mutants. Both Fringe-dependent and Fringe-independent Notch pathways are affected, and both glycosylation and proteolytic maturation of Notch are defective in mutant larvae. The results suggest that changes in nucleotide–sugar levels can differently affect Wingless and two distinct aspects of Notch signalling.
- Genetic Strain Research Center, National Institute of Genetics, Mishima, Shizuoka-ken 411-8540, Japan
- Department of Genetics, Graduate University for Advanced Studies, Mishima, Shizuoka-ken 411-8540, Japan
- Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoken), Bunkyo-ku, Tokyo 113-8613, Japan
- Division of Analytical Sciences, Faculty of Pharmaceutical Sciences, Chiba University, Inage, Chiba 263-8522, Japan
- RIKEN Center for Developmental Biology, Mishima, Shizuoka-ken 411-8540, Japan
- Present addresses: Laboratory of Molecular Biology, Medical Research Council, Hills Road, Cambridge CB2 2QH, UK (S.G.); Department of Applied Chemistry, Kogakuin University, Shinjuku-ku, Tokyo 163-8677, Japan (S.H.)
Correspondence to: Satoshi Goto1,2,6 e-mail: sgoto@mrc-lmb.cam.ac.uk
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