Table of contents


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Editorial

Deep impact? pE171

doi:10.1038/35087096


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Letters to Editor

Which Ras rides the raft? pE172

Michael A. White & Richard G. W. Anderson

doi:10.1038/35087098


Which Ras rides the raft? - Reply pE172

Ian A Prior, Robert G Parton & John F Hancock

doi:10.1038/35087100


Does endo-symbiosis explain the origin of the nucleus? pE173

Anthony Poole & David Penny

doi:10.1038/35087102


Does endo-symbiosis explain the origin of the nucleus? pE173

Carmen Rotte & William Martin

doi:10.1038/35087104


Does endo-symbiosis explain the origin of the nucleus? - Reply ppE173 - E174

Takao Shinozawa, Tokumasa Horiike & Kazuo Hamada

doi:10.1038/35087106


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Commentary

DNA microarrays and beyond: completing the journey from tissue to cell ppE175 - E178

Jason C. Mills, Kevin A. Roth, Ross L. Cagan & Jeffrey I. Gordon

doi:10.1038/35087108

For the cell biologist, identifying changes in gene expression using DNA microarrays is just the start of a long journey from tissue to cell. We discuss how chip users can first filter noise (false-positives) from daunting microarray datasets. Combining laser capture microdissection with real-time polymerase chain reaction and reverse transcription is a helpful follow-up step that allows expression of selected genes to be quantified in populations of recovered cells. The voyage from chip to single cell can be completed using sensitive new in situ hybridization and immunohistochemical methods based on tyramide signal amplification.


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News and Views

PX domains: attracted by phosphoinositides ppE179 - E182

Anne Simonsen & Harald Stenmark

doi:10.1038/35087112

Phosphoinositides recruit proteins to distinct intracellular membranes. Now, the Phox homology (PX) domain, an evolutionarily conserved protein domain whose function has so far been elusive, has been demonstrated to bind phosphoinositides. The interactions of PX-domain-containing proteins with specific phosphoinositides are critical for cellular activities such as microbial killing and membrane trafficking.


Getting activated with poly-ubiquitination pE181

Valerie Depraetere

doi:10.1038/35087116


The origin of CDK regulation ppE182 - E184

Anatoliy Li & J. Julian Blow

doi:10.1038/35087119

Cip/Kip proteins that inhibit cyclin-dependent kinase 2 (Cdk2) restrain the initiation of DNA replication. Degradation of a Xenopus Kip1 orthologue, Xic1, is dependent on its recruitment to replication origins. This ensures that activation of Cdk2 and subsequent initiation of replication is co-ordinately regulated at, and localized to, replication origins.


All for one and one for all pE184

Sarah Greaves

doi:10.1038/35087123


Signalling and endocytosis: Wnt breaks down on back roads ppE185 - E186

Matthew Scott

doi:10.1038/35087126

How are Wnt signals made effective in only the right times and places? New studies of the asymmetrical influences of Wnt signals originating from a source show the importance of differential signal degradation and regulated endocytosis.


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Book Reviews

A Primer to proliferation pE187

Michael Brandeis reviews Principles of Cell Proliferation by

doi:10.1038/35087129


Quo vadis with the Q tracts pE188

F. Ulrich Hartl & Peter Breuer review Glutamine Repeats and Neurodegenerative Diseases: Molecular Aspects by

doi:10.1038/35087132


Getting down to basics with actin pE189

Henry Higgs reviews Molecular Interactions of Actin: Actin Structure and Actin-Binding Proteins by

doi:10.1038/35087135


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Technology Review

Navigating gene expression using microarrays — a technology review ppE190 - E195

Almut Schulze & Julian Downward

doi:10.1038/35087138


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Meeting Report

Greasing the wheels of secretory transport ppE196 - E198

Jeremy W. Thorner

doi:10.1038/35087142


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Articles

SV2 modulates the size of the readily releasable pool of secretory vesicles pp691 - 698

Tao Xu & Sandra M. Bajjalieh

doi:10.1038/35087000


ActA and human zyxin harbour Arp2/3-independent actin-polymerization activity pp699 - 707

Julie Fradelizi, Vincent Noireaux, Julie Plastino, Bernadette Menichi, Daniel Louvard, Cécile Sykes, Roy M. Golsteyn & Evelyne Friederich

doi:10.1038/35087009


TGF-beta-induced apoptosis is mediated by the adapter protein Daxx that facilitates JNK activation pp708 - 714

Riki Perlman, William P. Schiemann, Mary W. Brooks, Harvey F. Lodish & Robert A. Weinberg

doi:10.1038/35087019


Triggering ubiquitination of a CDK inhibitor at origins of DNA replication pp715 - 722

Laura Furstenthal, Craig Swanson, Brett K. Kaiser, Adam G. Eldridge & Peter K. Jackson

doi:10.1038/35087026


mDia mediates Rho-regulated formation and orientation of stable microtubules pp723 - 729

Alexander F. Palazzo, Tiffani A. Cook, Arthur S. Alberts & Gregg G. Gundersen

doi:10.1038/35087035



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Brief Communications


Oncogenes in Ras signalling pathway dictate host-cell permissiveness to herpes simplex virus 1 pp745 - 750

Faris Farassati, An-Dao Yang & Patrick W. K. Lee

doi:10.1038/35087061


Nicastrin binds to membrane-tethered Notch pp751 - 754

Fusheng Chen, Gang Yu, Shigeki Arawaka, Masaki Nishimura, Toshitaka Kawarai, Haung Yu, Anurag Tandon, Agnes Supala, You Qiang Song, Ekaterina Rogaeva, Paul Milman, Christine Sato, Cong Yu, Christopher Janus, Julie Lee, Lixin Song, Lili Zhang, Paul E. Fraser & P. H. St George-Hyslop

doi:10.1038/35087069


The Eps15 C. elegans homologue EHS-1 is implicated in synaptic vesicle recycling pp755 - 760

Anna Elisabetta Salcini, Massimo Antonio Hilliard, Assunta Croce, Salvatore Arbucci, Paola Luzzi, Carlo Tacchetti, Laurie Daniell, Pietro De Camilli, Pier Giuseppe Pelicci, Pier Paolo Di Fiore & Paolo Bazzicalupo

doi:10.1038/35087075


Mammalian STAG3 is a cohesin specific to sister chromatid arms in meiosis I pp761 - 766

Ignacio Prieto, José A. Suja, Nieves Pezzi, Leonor Kremer, Carlos Martínez-A., Julio S. Rufas & José L. Barbero

doi:10.1038/35087082


Reproducible but dynamic positioning of DNA in chromosomes during mitosis pp767 - 770

Steffen Dietzel & Andrew S. Belmont

doi:10.1038/35087089


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