Article abstract
Nature Cell Biology 3, 567 - 572 (2001)
Published online: 14 May 2001 | doi:10.1038/35078543
Rme-1 regulates the distribution and function of the endocytic recycling compartment in mammalian cells
Sharron X. Lin1,3, Barth Grant3,2, David Hirsh2 & Frederick R. Maxfield1
Abstract
RME-1 is an Eps15-homology (EH)-domain protein that was identified in a genetic screen for endocytosis genes in Caenorhabditis elegans. When expressed in a CHO cell line, the worm RME-1 protein and a mouse homologue are both associated with the endocytic recycling compartment. Here we show that expression of a dominant-negative construct with a point mutation near the EH domain results in redistribution of the endocytic recycling compartment and slowing down of transferrin receptor recycling. The delivery of a TGN38 chimaeric protein to the trans-Golgi network is also slowed down. The function of Rme-1 in endocytic recycling is evolutionarily conserved in metazoans as shown by the protein's properties in C. elegans.
- Department of Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, New York 10021, USA
- Department of Biochemistry and Molecular Biophysics, Columbia University, College of Physicians and Surgeons, New York, New York 10032, USA
- These authors contributed equally to this work.
Correspondence to: Frederick R. Maxfield1 e-mail: frmaxfie@mail.med.cornell.edu
