Article abstract
Nature Cell Biology 3, 134 - 139 (2001)
Published online: 11 January 2001 | doi:10.1038/35055033
Interphase movements of a DNA chromosome region modulated by VP16 transcriptional activator
Tudorita Tumbar1 & Andrew S. Belmont1
Abstract
We examined changes in intranuclear chromosome positioning induced by a transcriptional activator in a simple experimental system. Targeting the VP16 acidic activation domain (AAD) to an engineered chromosome site resulted in its transcriptional activation and redistribution from a predominantly peripheral to a more interior nuclear localization. Direct visualization in vivo revealed that the chromosome site normally moves into the nuclear interior transiently in early G1 and again in early S phase. In contrast, VP16 AAD targeting induced this site's permanent interior localization in early G1. A single transcriptional activator therefore can modify the cell-cycle-dependent programme of intranuclear positioning of chromosome loci.
- Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, Room B107 C&LSL, 601 South Goodwin Avenue, Urbana, Illinois 61801, USA
Correspondence to: Andrew S. Belmont1 e-mail: asbel@uiuc.edu
